ADAPTATION OF BERGMAN AND LOXLEY TECHNIQUE FOR HYDROXYPROLINE DETERMINATION TO AUTOANALYZER AND ITS USE IN DETERMINING PLASMA HYDROXYPROLINE IN DOMESTIC FOWL
ADAPTATION OF BERGMAN AND LOXLEY TECHNIQUE FOR HYDROXYPROLINE DETERMINATION TO AUTOANALYZER AND ITS USE IN DETERMINING PLASMA HYDROXYPROLINE IN DOMESTIC FOWL
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DOI：
10.1039/an9709500596
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发表时间：
1970-01-01
期刊：
影响因子：
4.2
通讯作者：
BURNES, AB
中科院分区：
文献类型：
--
作者：
BANNISTER, DW;BURNES, AB
本文介绍了羟脯氨酸测定的手动技术在自动分析仪上的应用。该方法可用于测定2.6%以内的标准溶液，并可处理高达10 µg ml-1的连续浓度差。最佳准确度的浓度范围为2.5至15.0 µg ml-1。本文介绍了该技术在家禽血浆羟脯氨酸水平研究中的应用，并与同位素稀释法进行了比较，结果表明该技术无明显干扰。
The adaptation of a manual technique for hydroxyproline determination to the AutoAnalyzer is described. The method can be used to determine standard solutions to within 2·6 per cent. and to deal with successive concentration differences of up to 10 µg ml–1. The concentration range for optimum accuracy is 2·5 to 15·0 µg ml–1. Application of the technique to the study of plasma hydroxyproline levels in the domestic fowl is described, and comparison with isotope-dilution analysis demonstrates the lack of gross interference.