The distance between S1, S21, and the 3' end of 16S RNA in 30S ribosomal subunits. The effect of poly(uridylic acid) and 50S subunits on these distances.
The distance between S1, S21, and the 3' end of 16S RNA in 30S ribosomal subunits. The effect of poly(uridylic acid) and 50S subunits on these distances.
复制标题
30S 核糖体亚基中 S1、S21 和 16S RNA 3 端之间的距离。
DOI：
10.1111/j.1432-1033.1984.tb08280.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Hardesty,B
中科院分区：
文献类型：
--
作者：
Odom,OW;Dabbs,ER;Dionne,C;Müller,M;Hardesty,B
通过非辐射能量转移测定大肠杆菌30S核糖体亚基中S1或S21的半胱氨酸硫醇与16S RNA 3'端共价连接的探针之间的表观距离为：S21-16S RNA，5.1 nm； S21–S1，6.9 nm； S1–16S RNA，6.8 nm。聚尿苷酸与 30S 亚基的结合导致 S1 和 16S RNA 或 S21 和 16S RNA 之间的表观距离分别增加超过 1.2 nm 和 0.5 nm，但对 S1-S21 距离影响很小或没有影响。 50S 亚基的结合导致 S21-16S RNA 和 S21-S1 距离分别明显增加 1.0 nm 和 0.8 nm，但对 S1-16S-RNA 距离影响很小或没有影响。
The apparent distances between probes covalently attached to the cysteine thiols of S1 or S21 and the 3′ end of 16S RNA inEscherichia coli30S ribosomal subunits were determined by non‐radiative energy transfer to be: S21–16S RNA, 5.1 nm; S21–S1, 6.9 nm; S1–16S RNA, 6.8 nm. Binding of poly(uridylic acid) to 30S subunits causes the apparent distances between S1 and 16S RNA or S21 and 16S RNA to increase by more than 1.2 nm and 0.5 nm, respectively, but has little or no effect on the S1–S21 distance. Binding of 50S subunits causes an apparent increase in the S21 –16S RNA and S21–S1 distances by 1.0 nm and 0.8 nm, respectively, but has little or no effect on the S1–16S‐RNA distance.