Effects of fasting on physiologically pulsatile insulin release in healthy humans
Effects of fasting on physiologically pulsatile insulin release in healthy humans
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DOI：
10.2337/diabetes.51.2007.s255
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发表时间：
2002-02-01
期刊：
影响因子：
7.7
通讯作者：
Porksen, N
中科院分区：
文献类型：
--
作者：
Juhl, C;Grofte, T;Porksen, N
胰岛素作为叠加在基础释放上的分泌爆发释放。分泌爆发的总体贡献最近被量化为至少7596，并且胰岛素分泌的主要调节是通过扰动胰岛素释放的量和这些分泌爆发的频率。将胰岛素输送到循环中的模式似乎对胰岛素作用很重要，因此改变胰岛素释放模式的生理条件可能通过该机制影响胰岛素作用。为了评估禁食改变胰岛素释放量以及脉冲式胰岛素分泌的频率、幅度和总体贡献的机制，我们使用经验证的去卷积模型来检查7名健康受试者在禁食10和58小时期间的脉冲式胰岛素分泌。受试者在葡萄糖输注（2.5 mg . kg（-1）。min（-1））。我们发现，脉冲式胰岛素释放模式得以保留，并且在禁食时，通过减少胰岛素释放量来调节整体胰岛素释放以满足需求（10.1 +/- 1.7 vs. 16.0 +/- 3.2 pmol/l/脉冲，P < 0.05），但胰岛素分泌爆发的频率相似（6.3 +/- 0.4 vs. 6.1 +/- 0.4 min/脉冲）。在两种状态下，葡萄糖输注均引起量（100-200%）和频率（100- 20%）增加（P < 0.05）。葡萄糖浓度增加对脉冲频率的影响似乎在体内与体外脉冲胰岛素分泌不同，可能表明存在葡萄糖敏感性起搏器，其启动协调的分泌爆发。长期空腹时胰岛素/C肽比值增加（6.0 vs. 9.1%，P 0.01）表明胰岛素释放模式的变化可能伴随肝脏胰岛素提取的变化。糖尿病51（增刊1）：5255- 5257，2002。
Insulin is released as secretory bursts superimposed on basal release. The overall contribution of secretory bursts was recently quantified as at least 7596, and the main regulation of insulin secretion is through perturbation of the amount of insulin released and the frequency of these secretory bursts. The mode of delivery of insulin into the circulation seems important for insulin action, and therefore physiological conditions that alter the pattern of insulin release may affect insulin action through this mechanism. To assess the mechanisms by which fasting changes the amount of insulin released and the frequency, amplitude, and overall contribution of pulsatile insulin secretion, we used a validated deconvolution model to examine pulsatile insulin secretion during 10 and 58 h of fasting in seven healthy subjects. The subjects were studied for 75 min before (0-75 min) and 75 min during (115-190 min) a glucose infusion (2.5 mg . kg(-1) . min(-1)). Wee found that the pulsatile insulin release pattern was preserved and that, at fasting, overall insulin release is adjusted to needs by a reduced amount of insulin released (10.1 +/- 1.7 vs. 16.0 +/- 3.2 pmol/l/pulse, P < 0.05) but similar frequency (6.3 +/- 0.4 vs. 6.1 +/- 0.4 min/pulse) of the insulin secretory bursts. In both states, glucose infusion caused an increase (P < 0.05) in amount (100-200%) and frequency (∼20%). The impact of increased glucose concentration on pulse frequency seems distinct for in vivo versus in vitro pulsatile insulin secretion and may indicate the presence of a glucose-sensitive pacemaker, which initiates the coordinated secretory bursts. Increased insulin/C-peptide ratio at long-term fasting (6.0 vs. 9.1%, P < 0.01) indicates that the changes in insulin release patterns may be accompanied by changes in hepatic insulin extraction. Diabetes 51 (Suppl. 1): 5255-5257,2002.