Alterations of retinoblastoma protein and p16INK4 protein expression in extrahepatic bile duct carcinomas.
Alterations of retinoblastoma protein and p16INK4 protein expression in extrahepatic bile duct carcinomas.
复制标题
肝外胆管癌中视网膜母细胞瘤蛋白和 p16INK4 蛋白表达的变化。
作者：
X. Cui;A. Hui;X. Li;Y. Shi;T. Takayama;K. Hatakeyama;M. Makuuchi
背景/目的
人类癌症的发展是由细胞周期G1期调节因子功能障碍引起的。视网膜母细胞瘤蛋白和p16 INK 4是细胞周期调控与癌症之间最重要的联系。我们检查了34例肝外胆管癌中p16 INK 4和pRb的表达及其可能的预后相关性。
方法
免疫组化法检测pRb和p16 INK 4的表达。采用χ 2检验分析pRb和p16 INK 4表达与临床病理特征的关系，采用Log-rank检验分析生存期。
结果
2例（6%）肝外胆管癌pRb阴性，26例（76%）pRb过表达，6例（18%）中度表达。22例（65%）肿瘤p16 INK 4阴性，12例（35%）p16 INK 4阳性。pRb阴性或过表达与肿瘤进展（P = 0.004）和TNM分期（P = 0.009）显著相关。pRb和p16 INK 4表达的改变与患者预后无关。
结论
pRb和p16 INK 4表达的改变常与肝外胆管癌有关，pRb的异常表达与肿瘤的进展密切相关。
BACKGROUND/AIMS
Human cancer development results from dysfunction of G1-phase regulators of the cell cycle. Retinoblastoma protein and p16INK4 are the most essential links between cell cycle control and cancer. We examined the expression of p16INK4 and pRb and their possible prognostic relevance in 34 extrahepatic bile duct carcinomas.
METHODOLOGY
Expression of pRb and p16INK4 was determined using immunohistochemical techniques. Associations between expression of pRb and p16INK4 and the clinicopathological features were analyzed by using the chi 2 test and survival analysis was performed by Log-rank test.
RESULTS
Two (6%) extrahepatic bile duct carcinomas were pRb negative, 26 (76%) showed pRb overexpression, and 6 (18%) demonstrated moderate expression. Twenty-two (65%) tumors were p16INK4 negative and 12 (35%) were p16INK4-positive. Cases with pRb-negative or pRb overexpression were significantly correlated with tumor progression (P = 0.004) and TNM stage (P = 0.009). Alterations in pRb and p16INK4 expression did not correlate with patient outcome.
CONCLUSIONS
Alterations of pRb and p16INK4 expression are frequently involved in extrahepatic bile duct carcinomas, and that aberrant pRb expression significantly associates with tumor progression.