Thiazinogeldanamycin, a New Geldanamycin Derivative Produced by Streptomyces hygroscopicus 17997
Thiazinogeldanamycin, a New Geldanamycin Derivative Produced by Streptomyces hygroscopicus 17997
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Thiazinogeldanamycin，吸水链霉菌 17997 产生的新型格尔德霉素衍生物
DOI：
10.4014/jmb.1011.11006
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发表时间：
2011-06-01
影响因子：
2.8
通讯作者：
Wang, Yiguang
中科院分区：
文献类型：
--
作者：
Ni Siyang;Wu, Linzhuan;Wang, Yiguang
从吸水链霉菌17997发酵液中分离得到一个新的格尔德霉素衍生物。其化学结构经LC-MS、硫分析和NMR确证为噻嗪格尔德霉素。半胱氨酸的添加到发酵培养基中显着刺激噻嗪格尔德霉素的生产水平，表明半胱氨酸作为噻嗪格尔德霉素生产的前体。虽然对HepG 2癌细胞的细胞毒性降低，但噻嗪格尔德霉素表现出改善的水溶性和光稳定性。噻嗪格尔德霉素可能是迄今为止第一个以GDM为前体的天然GDM衍生物。它的出现也清楚地表明，一个适当的发酵终点是至关重要的最大生产GDM的吸水链霉菌17997。
A new geldanamycin (GDM) derivative was discovered and isolated from the fermentation broth of Streptomyces hygroscopicus 17997. Its chemical structure was elucidated as thiazinogeldanamycin by LC-MS, sulfur analysis, and NMR. The addition of cysteine to the fermentation medium significantly stimulated the production level of thiazinogeldanamycin, suggesting cysteine as a precursor of thiazinogeldanamycin production. Although showing a decreased cytotoxicity against HepG2 cancer cells, thiazinogeldanamycin exhibited an improved water solubility and photostability. Thiazinogeldanamycin may represent the first natural GDM derivative characterized so far that uses GDM as its precursor. Its appearance also clearly indicates that an appropriate end-point of fermentation is of critical importance for the maximal production of GDM by Streptomyces hygroscopicus 17997.