FATE OF BACTERIA INJECTED INTO NAIVE AND IMMUNIZED LARVAE OF THE TOBACCO HORNWORM MANDUCA-SEXTA
FATE OF BACTERIA INJECTED INTO NAIVE AND IMMUNIZED LARVAE OF THE TOBACCO HORNWORM MANDUCA-SEXTA
复制标题
DOI：
10.1016/0022-2011(83)90238-0
复制
发表时间：
1983-01-01
影响因子：
3.4
通讯作者：
DRAKE, DR
中科院分区：
文献类型：
--
作者：
DUNN, PE;DRAKE, DR
M. Sexta幼虫感染几种革兰氏阴性菌进行了检查。在任何试验剂量下，注射大肠杆菌D31均未导致死亡。粘质沙雷氏菌和铜绿假单胞菌ATCC 9027是中等致病性的，并且在超过106个细菌/昆虫的剂量注射后长时间引起低死亡率。铜绿假单胞菌P11-1是有毒的，在超过103个细菌/昆虫的剂量下杀死所有注射的昆虫。P11-1治疗与死亡之间的时间具有剂量依赖性。注射的铜绿假单胞菌ATCC 9027和P11-1活细胞以及E. coliD 31感染未免疫的M. sexta（细菌清除）。在所有检测剂量下，注射后第1小时内，所有3种细菌的活细胞浓度均显著降低。这种浓度的初始降低之后是应变和剂量依赖性多相结果。在初始减少后，细菌浓度立即有一段时间没有变化，随后是活细菌的完全消除或细菌增殖和宿主死亡。还在用铜绿假单胞菌和大肠杆菌两种菌株的白蛋白免疫的幼虫中检查了铜绿假单胞菌（P11-1和ATCC 9027）的活细胞的命运。coli D31。在这些免疫的昆虫中，所有菌株的活细菌浓度的初始降低之后是随后降低至低水平的细菌，其维持至少24小时。E. coliD 31与作为抗铜绿假单胞菌P11-1的免疫原的同源性Escherichin相当。在所有菌株中观察到的细菌浓度的初始降低是注射部位或附近形成结节的结果。随后的控制细菌繁殖的幼稚和免疫幼虫可能反映了有效的吞噬血细胞和细菌的敏感性诱导的体液杀菌因子。铜绿假单胞菌P11-1在幼稚昆虫中的致病性可能是由于该菌株对吞噬作用的相对抗性，从而导致在杀菌因子出现之前的增殖和细菌分泌的细胞毒素的积累。
The susceptibility of M. sexta larvae to infection by several Gram-negative bacteria was examined. Injected Escherichia coli D31 caused no mortality at any dose tested. Serratia marcescens and Pseudomonas aeruginosa ATCC 9027 were moderately pathogenic and caused low mortality at long times after injection of doses exceeding 106 bacteria/insect P. aerguinosa P11-1 was virulent, killing all injected insects at doses exceeding 103 bacteria/insect. The period between treatment with P11-1 and death was dose-dependent. The fate of injected viable cells of P. aeruginosa ATCC 9027 and P11-1 and of E. coli D31 was examined in naive (nonimmunized) larvae of M. sexta (bacterial clearance). The concentrations of viable cells of all 3 bacteria were reduced significantly during the 1st h following injection at all doses tested. This initial reduction in concentration was followed by a strain and dose-dependent multiphasic outcome. Immediately after the initial reduction there was a period of no change in bacterial concentration followed by either complete elimination of viable bacteria or bacterial multiplication and host death. The fate of viable cells of P. aeruginosa (P11-1 and ATCC 9027) was also examined in larvae immunized with bacterins of both strains of P. aeruginosa and of E. coli D31. In these immunized insects, the initial reduction in concentration of viable bacteria of all strains was followed by subsequent reductions to low levels of bacteria which were maintained for at least 24 h. Viable cells of E. coli D31 were comparable to homologous bacterin as an immunogen against P. aeruginosa P11-1. The initial reduction in bacterial concentration observed with all strains was a result of nodule formation at or near the injection site. Subsequent control of bacterial multiplication in naive and immunized larvae may reflect efficient phagocytosis by hemocytes and susceptibility of bacteria to induced humoral bactericidal factors. The pathogenicity of P. aeruginosa P11-1 in naive insects may be due to relative resistance of this strain to phagocytosis resulting in multiplication prior to the appearance of bactericidal factors and the accumulation of cytotoxins excreted by the bacterium.