Long-term survival and intracellular replication of Mycoplasma hominis in Trichomonas vaginalis cells:: Potential role of the protozoon in transmitting bacterial infection
Long-term survival and intracellular replication of Mycoplasma hominis in Trichomonas vaginalis cells:: Potential role of the protozoon in transmitting bacterial infection
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DOI：
10.1128/iai.73.2.1180-1186.2005
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发表时间：
2005-02-01
影响因子：
3.1
通讯作者：
Rappelli, P
中科院分区：
文献类型：
--
作者：
Dessì, D;Delogu, G;Rappelli, P
本课题组最近报道了阴道毛滴虫和人型支原体之间存在共生关系，这是第一个报道的两种专性人类病原体之间共生的例子。在这项工作中，我们研究了M的细胞定位。hominis在T.流浪汉通过使用庆大霉素保护试验、双重免疫荧光和共聚焦显微镜，我们获得了强有力的证据。人位于原生动物细胞内。5-溴脱氧尿苷掺入试验表明，位于细胞内的支原体积极合成DNA。结果表明，M.人具有进入毛滴虫细胞和在原生动物内复制的能力。这些发现表明，共生可能提供细菌，在人类感染，具有抵抗环境压力的能力，如宿主防御机制和药物治疗。
The existence of a symbiotic relationship between Trichomonas vaginalis and Mycoplasma hominis, which is the first reported example of symbiosis between two obligate human pathogens, has been recently reported by our research group. In this work, we examined the cellular location of M. hominis in respect to T. vaginalis. By using gentamicin protection assays, double immunofluorescence, and confocal microscopy, we obtained strong evidence that M. hominis is located within protozoan cells. 5-Bromodeoxyuridine incorporation assays showed that intracellularly located mycoplasmas actively synthesize DNA. Our results demonstrate that M. hominis has the capability of entering trichomonad cells and of replicating inside the protozoon. These findings suggest that symbiosis might provide the bacteria, during human infection, with the capability to resist to environmental stresses, such as host defense mechanisms and pharmacological therapies.