Assessment of ultraviolet-radiation-induced DNA damage within melanocytes in skin of different constitutive pigmentation
Assessment of ultraviolet-radiation-induced DNA damage within melanocytes in skin of different constitutive pigmentation
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DOI：
10.1111/bjd.12201
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发表时间：
2013-05-01
影响因子：
10.3
通讯作者：
Bernerd, F.
中科院分区：
文献类型：
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作者：
Del Bino, S.;Sok, J.;Bernerd, F.
背景黑色素瘤的发病率和色素紊乱与皮肤色素沉着的程度有关，但关于紫外线对不同成分色素沉着皮肤的黑素细胞的具体影响的数据很少。目的分析紫外线照射对不同肤色黑素细胞的DNA损伤作用。方法根据不同皮肤类型角度(ITA度)，将皮肤样本客观地分为浅、中、棕褐色和深色皮肤。样本暴露在剂量越来越大的太阳模拟辐射中。用环丁烷胸腺嘧啶二聚体(CPD)酪氨酸酶相关蛋白1双染色法检测黑素细胞DNA损伤。结果浅、中、棕褐色皮肤在最低剂量下可检测到黑素细胞中CPDS的积聚，且随剂量的增加而急剧增加。在估计的红斑等量剂量下，在棕褐色、中等和浅色皮肤类型中，大约80100%的黑素细胞对CPD呈阳性。相比之下，在深色和棕色皮肤类型中，在最高剂量下，只有大约15%的黑素细胞中发现了CPD。结论这项工作表明，就紫外线辐射引起的DNA损伤而言，来自结构性高色素皮肤类型的黑素细胞比来自较浅皮肤类型的黑素细胞受到的影响更小，即使是中等色素皮肤类型的黑素细胞。
Background Melanoma incidence and pigmentary disorders are known to be related to the degree of skin pigmentation, but few data exist on the specific impact of ultraviolet radiation (UVR) on melanocytes in skin of different constitutive pigmentation. Objectives To analyse UVR-induced DNA damage within melanocytes in different skin-colour types. Methods Skin samples were objectively classified into light, intermediate, tan, brown and dark skin according to their individual typology angle (degrees ITA), based on colorimetric parameters. Samples were exposed to increasing doses of solar simulated radiation. Detection of DNA damage specifically in melanocytes was achieved by cyclobutane thymine dimer (CPD)tyrosinase-related protein 1 double staining. Results For light, intermediate and tan skin, accumulation of CPDs in melanocytes was detected at the lowest dose, with a steep increase with dose. At estimated erythemally equivalent doses, around 80100% of melanocytes were positive for CPDs in tan, intermediate and light skin types. In contrast, in dark and brown skin types, CPDs were found in only approximately 15% of melanocytes at the highest dose. Conclusions This work demonstrates that melanocytes from constitutively highly pigmented skin types are less impacted in terms of UVR-induced DNA damage than those from lighter skin types, even those that are moderately pigmented.