Characterization of glutamatergic VTA neural population responses to aversive and rewarding conditioning in freely-moving mice
Characterization of glutamatergic VTA neural population responses to aversive and rewarding conditioning in freely-moving mice
复制标题
自由活动小鼠中谷氨酸能 VTA 神经群对厌恶和奖励条件反应的表征
DOI：
10.1016/j.scib.2019.05.005
复制
发表时间：
2019-08-30
期刊：
影响因子：
18.9
通讯作者：
Wang, Liping
中科院分区：
文献类型：
--
作者：
Montardy, Quentin;Zhou, Zheng;Wang, Liping
中脑被盖区（VTA）是一个整合厌恶性和奖励性刺激的中脑结构，但对VTA神经元（VGluT 2）在这些功能中的作用知之甚少。VGluT 2索马的直接激活引起奖励行为，而其下游投射的激活引起厌恶行为。为了便于我们理解这些相互冲突的属性，我们记录了VTA（VGluT 2+）神经元的钙信号，使用纤维光度法在VGluT 2-cre小鼠，以研究这个人口是如何招募的厌恶和奖励刺激，无论是在无条件和条件协议。我们的研究结果表明，作为一个群体，VTA（VGluT 2+）神经元对无条件厌恶和无条件奖励刺激的反应相似。在厌恶和奖励条件反射过程中，CS诱发的反应逐渐增加，而US诱发的反应保持稳定。检索24小时后，空调，在此期间，小鼠只收到CS介绍，导致腹侧被盖区（VGluT 2+）神经元强烈响应CS介绍和预期的美国，但只厌恶条件。为了帮助理解基于VTA（VGluT 2+）神经元网络的这些差异，使用霍乱毒素B（CT B）和狂犬病病毒研究VTA（VGluT 2+）神经元的输入和输出。基于我们的研究结果，我们提出，不同的VTA（VGluT 2+）神经元反应的厌恶和奖励条件可能部分是由于存在的VTA（VGluT 2+）亚群，其特征在于它们的连接。(C)中国科学出版社.爱思唯尔公司、科学中国出版社出版。
The Ventral Tegmental Area (VTA) is a midbrain structure known to integrate aversive and rewarding stimuli, but little is known about the role of VTA glutamatergic (VGluT2) neurons in these functions. Direct activation of VGluT2 soma evokes rewarding behaviors, while activation of their downstream projections evokes aversive behaviors. To facilitate our understanding of these conflicting properties, we recorded calcium signals from VTA(VGluT2+) neurons using fiber photometry in VGluT2-cre mice to investigate how this population was recruited by aversive and rewarding stimulation, both during unconditioned and conditioned protocols. Our results revealed that, as a population, VTA(VGluT2+) neurons responded similarly to unconditioned-aversive and unconditioned-rewarding stimulation. During aversive and rewarding conditioning, the CS-evoked responses gradually increased across trials whilst the US-evoked response remained stable. Retrieval 24 h after conditioning, during which mice received only CS presentation, resulted in VTA(VGluT2+) neurons strongly responding to CS presentation and to the expected-US but only for aversive conditioning. To help understand these differences based on VTA(VGluT2+)neuronal networks, the inputs and outputs of VTA(VGluT2+) neurons were investigated using Cholera Toxin B (CTB) and rabies virus. Based on our results, we propose that the divergent VTA(VGluT2+) neuronal responses to aversion and reward conditioning may be partly due to the existence of VTA(VGluT2+) subpopulations that are characterized by their connectivity. (C) 2019 Science China Press. Published by Elsevier B.V. and Science China Press.