Enhanced production of ansamitocin P-3 by addition of Mg2+ in fermentation of Actinosynnema pretiosum
Enhanced production of ansamitocin P-3 by addition of Mg2+ in fermentation of Actinosynnema pretiosum
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DOI：
10.1016/j.biortech.2011.08.031
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发表时间：
2011-11-01
影响因子：
11.4
通讯作者：
Zhong, Jian-Jiang
中科院分区：
文献类型：
--
作者：
Jia, Yongliang;Zhong, Jian-Jiang
二价金属离子（即，研究了Mn ~（2+）、Mg ~（2+）、Zn ~（2+）、Cu ~（2+）和Co ~（2+）对放射丝胞藻（Actinosynnema pretiosum）在含有农业工业废渣的培养基中发酵生产抗癌安丝菌素P-3（AP-3）的影响，发现Mg ~（2+）的效果最好。在最佳Mg ~（2+）添加条件下，AP-3的最高产量可达85 mg/L，是对照的3.0倍。甲基丙二酰辅酶A羧基转移酶（MCT）和甲基丙二酰辅酶A羧化酶（MCM）活性增强。两种前体物质丙二酰辅酶A和甲基丙二酰辅酶A的含量均低于对照。这项工作表明，Mg 2+的添加是一个简单而有效的策略，通过调节酶的活性和池的前体增加AP-3的生产。所获得的信息有助于其大规模高效生产。(C)2011爱思唯尔有限公司版权所有。
The effect of divalent metal ions (i.e., Mn2+, Mg2+, Zn2+, Cu2+, and Co2+) on the production of anticancer ansamitocin P-3 (AP-3) by submerged cultures of Actinosynnema pretiosum in medium containing agro-industrial residues was investigated, and Mg2+ was found to be the most effective. Under the optimal condition of Mg2+ addition, the maximal AP-3 production titer reached 85 mg/L, which was 3.0-fold that of the control. The activities of methylmalonyl-CoA carboxyltransferase (MCT) and methylmalonyl-CoA mutase (MCM) were enhanced. The content of two precursors, malonyl-CoA and methylmalonyl-CoA, was lower than that of control. This work demonstrates that Mg2+ addition is a simple and effective strategy for increasing AP-3 production through the regulation of enzyme activity and pools of precursors. The information obtained can be helpful to its efficient production on large scale. (C) 2011 Elsevier Ltd. All rights reserved.