Two techniques of ostracod (Ostracoda, Crustacea) extraction from organic detritus-rich sediments
Two techniques of ostracod (Ostracoda, Crustacea) extraction from organic detritus-rich sediments
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DOI：
10.1016/j.limno.2012.12.001
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发表时间：
2013-06
期刊：
影响因子：
1.7
通讯作者：
A. Szlauer-Łukaszewska;T. Radziejewska
中科院分区：
文献类型：
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作者：
A. Szlauer-Łukaszewska;T. Radziejewska
从富含碎屑的海底沉积物中提取介形虫(介形虫，甲壳纲)的两种简单技术：沉积物曝气和沉积环境恶化，在一条大型低地河流(波兰西部的奥德拉河)采集的样品上进行了测试。应用于保存样品的曝气技术，包括将空气鼓泡到覆盖着水的沉积物样品中。这些动物会附着在气泡表面，因此它们会被带到水层并附着在水面上。环境恶化技术应用于未保存的沉积物(假设含有活的介形虫)，包括将一个装满沉积物的沙漏放在装满水的容器中过夜。到了晚上，小型底栖动物会离开沉积物，移到水相，在那里很容易收集到它们。与手工分选相比，两种技术都大大减少了提取时间(曝气时平均减少约87%，变质时平均减少约70%)。就效率而言，这两种技术的表现都取决于物种。研究发现，曝气技术对光滑甲壳物种具有选择性，由于研究区域内光滑甲壳物种的优势，曝气技术取得了令人满意的效果(大多数情况下提取效率超过80%)。变质技术也显示出物种间提取适应性的显著差异；那些被认为对退化条件耐受性较差且具有游动刚毛的物种将优先被提取。在这项研究中，介形类组合以能够游泳的物种为主，对环境恶化的耐受性较低，因此，在大多数情况下，这项技术的效率超过60%。
The performance of two simple techniques of ostracod (Ostracoda, Crustacea) extraction from detritus-rich bottom sediment: sediment aeration and sedimentary environment deterioration, was tested on samples collected in a large lowland river (River Odra, western part of Poland). The aeration technique, applied to preserved samples, involved bubbling air into the sediment sample overlain with water. The animals would adhere to the bubble surface, whereby they would be brought to the water layer and cling to the water surface. The environment deterioration technique, applied to unpreserved sediment (assumed to contain live ostracods), involved placing a sediment-filled hour glass in a water-filled vessel overnight. At night, meiobenthic animals would leave the sediment and move to the aqueous phase from which they could be easily collected. Compared to hand sorting, both techniques substantially (by an average of about 87% in aeration and about 70% in deterioration) reduced extraction time. In terms of efficiency, performance of both techniques was species-dependent. The aeration technique was found to be selective towards smooth-carapace species, and performed satisfactorily (extraction efficiency of more than 80% in most cases) due to the preponderance of smooth-carapace species in the area of study. The deterioration technique, too, showed significant between-species differences in amenability to extraction; those species supposedly less tolerant to deteriorating conditions and possessing swimming setae would be preferentially extracted. The ostracod assemblage in this study was dominated by species capable of swimming and supposed to have low tolerance to deteriorating conditions, for which reason the technique, in most cases, performed with efficiency exceeding 60%.