Isolation and characterization of aniline-degrading Rhodococcus sp. strain AN5
Isolation and characterization of aniline-degrading Rhodococcus sp. strain AN5
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DOI：
10.1080/10934520701629674
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发表时间：
2007-11
期刊：
影响因子：
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通讯作者：
Rensheng Zhuang;W. Zhong;Jun Yao;Huilun Chen;Lin Tian;Yong Zhou;Fei Wang;E. Bramanti;G. Záray
中科院分区：
文献类型：
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作者：
Rensheng Zhuang;W. Zhong;Jun Yao;Huilun Chen;Lin Tian;Yong Zhou;Fei Wang;E. Bramanti;G. Záray
以苯胺为唯一碳源和能量来源，在中国江苏省南京市南京化工厂的低盐培养基（MSM）上通过选择性富集培养分离出一株纯培养菌。经16S rRNA基因序列和形态生理特征分析，该菌株为红球菌属成员，命名为菌株AN5。其生物降解苯胺的最佳条件为30℃（pH 7.0）。由于苯胺不溶于水，采用分光光度法和微量热法研究了菌株AN5在不同苯胺浓度下的生长情况。菌株AN5不仅以苯胺为唯一的碳源和能量来源，还能降解苯酚、苯甲酸和萘。葡萄糖、蛋白胨和硫酸铵作为其二次碳源和氮源时，对苯胺的生物降解没有积极影响。这些发现对菌株AN5在工业废水净化中的应用具有一定的参考价值。
A pure culture using aniline as a sole source of carbon and energy was isolated by selective enrichment culturing on a minimum salt medium (MSM) from the Nanjing Chemical Plant, Nanjing, Jiangsu Province, China. An analysis of the 16S rRNA gene sequence and morphological and physiological characteristics showed that this strain was a member of the genus Rhodococcus, and it was designated as strain AN5. Its optimal conditions for aniline biodegradation were 30°C (pH 7.0). Due to the water insolubility of aniline, growth of strain AN5 at various aniline concentrations was studied via spectrophotometry and microcalorimetry in different culture mediums. Strain AN5 not only utilized aniline as its sole carbon and energy resources, but also degraded phenol, benzoic acid and naphthalin. Glucose, peptone and ammonium sulfate, when utilized as its secondary carbon and nitrogen sources, had no active effect on the aniline biodegradation. Such findings would be valuable in the application of strain AN5 to depurate industrial waste water.