Differential impact of Lhx2 deficiency on expression of class I and class II odorant receptor genes in mouse
Differential impact of Lhx2 deficiency on expression of class I and class II odorant receptor genes in mouse
复制标题
DOI：
10.1016/j.mcn.2007.01.014
复制
发表时间：
2007-04-01
影响因子：
3.5
通讯作者：
Mombaerts, Peter
中科院分区：
文献类型：
--
作者：
Hirota, Junji;Omura, Masayo;Mombaerts, Peter
气味受体（OR）基因可分为两类：鱼类样I类OR基因和哺乳动物特异性II类OR基因。我们以前已经表明，Lhx2，一个LIM同源结构域蛋白，结合到小鼠M71，II类OR的启动子区中的同源结构域位点，并且Lhx2中的敲除突变排除了所有测试的II类OR基因（包括M71）的表达。在这里，我们报告说，大多数I类OR基因，这是在嗅上皮的背侧区域表达，仍然在Lhx2缺陷的胚胎中表达。有两个例外：两个I类OR基因，通常在更腹侧的区域表达，在Lhx2突变小鼠中不再表达。Lhx2在嗅觉感觉神经元中转录，与I类或II类OR基因的表达无关。因此，Lhx2的缺乏对I类和II类OR基因表达具有不同的影响。(c)2007年爱思唯尔公司All rights reserved.
Odorant receptor (OR) genes can be classified into two types: fish-like class I OR genes and mammalian-specific class II OR genes. We have previously shown that Lhx2, a LIM-homeodomain protein, binds to the homeodomain site in the promoter region of mouse M71, a class II OR, and that a knockout mutation in Lhx2 precludes expression of all tested class II OR genes including M71. Here, we report that most class I OR genes, which are expressed in a dorsal region of the olfactory epithelium, are still expressed in Lhx2-deficient embryos. There are two exceptions: two class I OR genes, which are normally expressed in a more ventral region, are no longer expressed in Lhx2 mutant mice. Lhx2 is transcribed in olfactory sensory neurons irrespective of expression of class I or class II OR genes. Thus, a deficiency of Lhx2 has a differential impact on class I and class II OR gene expression. (c) 2007 Elsevier Inc. All rights reserved.