An application of the micropipette technique to the measurement of the mechanical properties of cultured bovine aortic endothelial cells.
An application of the micropipette technique to the measurement of the mechanical properties of cultured bovine aortic endothelial cells.
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微量移液器技术在测量培养牛主动脉内皮细胞力学性能中的应用。
DOI：
10.1115/1.3138638
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Nerem,RM
中科院分区：
文献类型：
--
作者：
Sato,M;Levesque,MJ;Nerem,RM
用微吸管技术测定内皮细胞的力学性能。所用细胞取自牛主动脉内皮细胞，在本实验室进行培养。在无流动条件下，用胰酶或机械方法将融合单层内皮细胞从基质中分离出来，悬浮在改良的Dulbecco培养液中。在微吸管技术中，细胞的一部分在显微镜下被吸入微吸管的尖端，并通过照片测量变形。在这项研究中，所获得的数据是使用一个模型进行分析的，其中被认为是主要承压成分的细胞骨架元素被假设位于膜下皮质层中。根据对单个细胞表面不同区域的测量，发现分离的细胞具有几乎均匀的机械性能。然而，在加载和卸载过程中，压力与细胞变形之间的关系出现了滞后曲线。计算得到的胰酶分离细胞的弹性剪切模数是红细胞的10-20倍。机械分离的细胞的弹性系数大约是胰酶分离的细胞的两倍。传代时间，即细胞培养年龄，对胰酶分离的细胞的力学性质没有影响，但对机械分离的细胞有影响，年轻和年长的细胞都有一定程度的僵硬。
The mechanical properties of endothelial cells were measured using the micropipette technique. The cells employed were collected from bovine aortic endothelium and cultured in our laboratory. Endothelial cells from confluent monolayers under noflow conditions were detached from their substrate by trypsin or by a mechanical method and suspended in modified Dulbecco medium (MDM). In the micropipette technique, a part of the cell is aspirated into the tip of the micropipette under a microscope, and the deformation measured from a photograph. In this study, the data obtained were analyzed using a model where the cytoskeletal elements, which are considered to be the primary stress bearing components, are assumed to reside in a submembranous, cortical layer. Detached cells were found to have almost homogeneous mechanical properties based on measurements from different regions of the surface of a single cell. However, a hysteresis loop was observed in the relation between pressure and cell deformation during the loading and unloading processes. The calculated elastic shear moduli obtained for the trypsin-detached cells were as much as 10–20 times larger than those of a red blood cell. Mechanically-detached cells had moduli approximately twice that of the trypsin detached cells. Passage time, i.e., cell culture age, had no influence on the mechanical properties of the trypsin-detached cells, but did have an effect on the mechanically-detached cells, with both the younger and older cells being somewhat stiffer.