Removal of background signal from in situ data on the expression of segmentation genes in Drosophila.
Removal of background signal from in situ data on the expression of segmentation genes in Drosophila.
复制标题
从果蝇分段基因表达的原位数据中去除背景信号。
DOI：
10.1007/s00427-005-0472-2
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发表时间：
2005
影响因子：
2.4
通讯作者：
Reinitz,John
中科院分区：
文献类型：
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作者：
Myasnikova,Ekaterina;Samsonova,Maria;Kosman,David;Reinitz,John
在这里，我们提出了一种方法来消除非特异性背景信号的荧光定位表达模式ofDrosophilasegmentation基因。我们的算法去除背景信号带来的数据，以一个共同的标准形式与零背景，并消除系统误差的基因表达水平所造成的背景的存在。该方法是基于发现，报告在这里，即背景是很好地适合一个非常广泛的二维抛物面。抛物面是从胚胎中不表达给定基因的区域确定的，然后通过该抛物面将整个模式标准化以从整个胚胎中去除背景。实现该算法的软件可从作者处获得。
Here we present a method for the removal of nonspecific background signal from fluorescently localized expression patterns ofDrosophilasegmentation genes. Our algorithm for removal of background signal brings the data to a common standard form with zero background and removes systematic error in gene expression levels caused by the presence of background. The method is based on the discovery, reported here, that background is well fit by a very broad two-dimensional paraboloid. The paraboloid is determined from the area of the embryo in which a given gene is not expressed and the whole pattern is then normalized by this paraboloid to remove background from the entire embryo. The software implementing this algorithm is available from the authors.