Characterization of Vascular Disruption and Blood-Spinal Cord Barrier Permeability following Traumatic Spinal Cord Injury
Characterization of Vascular Disruption and Blood-Spinal Cord Barrier Permeability following Traumatic Spinal Cord Injury
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DOI：
10.1089/neu.2013.3034
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发表时间：
2014-03-15
影响因子：
4.2
通讯作者：
Fehlings, Michael G.
中科院分区：
文献类型：
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作者：
Figley, Sarah A.;Khosravi, Ramak;Fehlings, Michael G.
脊髓损伤(SCI)后会发生显著的血管改变，这有助于进行性的病理生理学。在本研究中，我们使用雌性Wistar大鼠(300-350g)和T6至T7的35g夹压损伤来表征脊髓损伤后随之而来的空间和时间血管变化。处死动物前，分别注射血管示踪染料(2%Evans Blue(EB)或异硫氰酸荧光素/番茄凝集素[FITC-LEA])，以评价血脊髓屏障(BSCB)的完整性或血管构筑。EB组织光谱分析显示BSCB在伤后24小时达到最大值，并持续到伤后5天(P<0.05)。
Significant vascular changes occur subsequent to spinal cord injury (SCI), which contribute to progressive pathophysiology. In the present study, we used female Wistar rats (300-350 g) and a 35-g clip-compression injury at T6 to T7 to characterize the spatial and temporal vascular changes that ensue post-SCI. Before sacrifice, animals were injected with vascular tracing dyes (2% Evans Blue (EB) or fluorescein isothiocyanate/Lycopersicon esculentum agglutinin [FITC-LEA]) to assess blood-spinal cord barrier (BSCB) integrity or vascular architecture, respectively. Spectrophotometry of EB tissue showed maximal BSCB disruption at 24 h postinjury, with significant disruption observed until 5 days postinjury (p