Characterization of EhaJ, a New Autotransporter Protein from Enterohemorrhagic and Enteropathogenic Escherichia coli.
Characterization of EhaJ, a New Autotransporter Protein from Enterohemorrhagic and Enteropathogenic Escherichia coli.
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EHAJ的表征，EHAJ是一种新的自动转运蛋白，来自肠菌和肠病性大肠杆菌。
DOI：
10.3389/fmicb.2011.00120
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发表时间：
2011
影响因子：
5.2
通讯作者：
Schembri MA
中科院分区：
文献类型：
--
作者：
Easton DM;Totsika M;Allsopp LP;Phan MD;Idris A;Wurpel DJ;Sherlock O;Zhang B;Venturini C;Beatson SA;Mahony TJ;Cobbold RN;Schembri MA
肠出血性大肠杆菌（EHEC）和肠致病性大肠杆菌。大肠埃希菌（EPEC）是大肠埃希菌的主要致病型。大肠杆菌，导致胃肠道疾病的潜在威胁生命的后遗症。虽然某些肠出血性大肠杆菌和EPEC毒力机制已被广泛研究，介导宿主定植的因素仍有待适当定义。在此之前，我们从原型EHEC菌株EDL 933中鉴定了4个基因（ehaA、ehaB、ehaC和ehaD），它们编码属于自转运蛋白（AT）家族的蛋白质。在这里，我们已经检查了这些基因的流行，以及其他几个AT编码基因，在收集的肠出血性大肠杆菌和EPEC菌株。我们发现，在肠出血性大肠杆菌和EPEC菌株的AT编码基因的互补是可变的，一些AT编码基因是非常普遍的。一个以前未知的AT编码基因，我们称之为ehaJ，被确定在12/44（27%）的肠出血性大肠杆菌和2/20（10%）的EPEC菌株。ehaJ基因紧邻编码推定的糖基转移酶（称为egtA）的基因。使用EhaJ特异性抗体的Western印迹分析表明EhaJ被EgtA糖基化。EhaJ在重组E.大肠杆菌菌株，揭示EhaJ位于细胞表面，并在egtA糖基转移酶基因的存在下，介导了强烈的生物膜形成在微量滴定板和流动细胞测定。EhaJ还介导了一系列细胞外基质蛋白的粘附，然而这不依赖于糖基化而发生。我们还表明，EhaJ在体外生长后在野生型EPEC菌株中表达。然而，删除ehaJ并没有显着改变其粘附或生物膜特性。总之，EhaJ是来自EPEC和EHEC的新的糖基化AT蛋白。EhaJ在致病性和定殖中的作用有待进一步研究。
Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) are diarrheagenic pathotypes of E. coli that cause gastrointestinal disease with the potential for life-threatening sequelae. While certain EHEC and EPEC virulence mechanisms have been extensively studied, the factors that mediate host colonization remain to be properly defined. Previously, we identified four genes (ehaA, ehaB, ehaC, and ehaD) from the prototypic EHEC strain EDL933 that encode for proteins that belong to the autotransporter (AT) family. Here we have examined the prevalence of these genes, as well as several other AT-encoding genes, in a collection of EHEC and EPEC strains. We show that the complement of AT-encoding genes in EHEC and EPEC strains is variable, with some AT-encoding genes being highly prevalent. One previously uncharacterized AT-encoding gene, which we have termed ehaJ, was identified in 12/44 (27%) of EHEC and 2/20 (10%) of EPEC strains. The ehaJ gene lies immediately adjacent to a gene encoding a putative glycosyltransferase (referred to as egtA). Western blot analysis using an EhaJ-specific antibody indicated that EhaJ is glycosylated by EgtA. Expression of EhaJ in a recombinant E. coli strain, revealed EhaJ is located at the cell surface and in the presence of the egtA glycosyltransferase gene mediates strong biofilm formation in microtiter plate and flow cell assays. EhaJ also mediated adherence to a range of extracellular matrix proteins, however this occurred independent of glycosylation. We also demonstrate that EhaJ is expressed in a wild-type EPEC strain following in vitro growth. However, deletion of ehaJ did not significantly alter its adherence or biofilm properties. In summary, EhaJ is a new glycosylated AT protein from EPEC and EHEC. Further studies are required to elucidate the function of EhaJ in colonization and virulence.
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影响因子：
3.1
作者：
Paton, AW;Srimanote, P;Paton, JC
通讯作者：
Paton, JC
影响因子：
3.1
作者：
Allsopp, Luke P.;Totsika, Makrina;Schembri, Mark A.
通讯作者：
Schembri, Mark A.
影响因子：
3.1
作者：
Kostakioti, M;Stathopoulos, C
通讯作者：
Stathopoulos, C
影响因子：
3.2
作者：
Sherlock, O;Dobrindt, U;Klemm, P
通讯作者：
Klemm, P
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL