IMMUNE DEPOSITS IN NORMAL SKIN OF PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS - RELATIONSHIP TO THE SERUM CAPACITY TO SOLUBILIZE IMMUNE-COMPLEXES
IMMUNE DEPOSITS IN NORMAL SKIN OF PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS - RELATIONSHIP TO THE SERUM CAPACITY TO SOLUBILIZE IMMUNE-COMPLEXES
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DOI：
10.1016/0090-1229(85)90047-9
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
DANIELI, G
中科院分区：
文献类型：
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作者：
GABRIELLI, A;CORVETTA, A;DANIELI, G
对134例各种结缔组织疾病患者的皮肤真皮-表皮交界处（DEJ）的免疫荧光沉积物（狼疮带试验或LBT）进行了评价。32例系统性红斑狼疮（SLE）中23例（71.8%）、53例类风湿性关节炎（RA）中3例（5.6%）、5例混合性结缔组织病（MCTD）中1例LBT阳性。在系统性硬化症和血管炎患者中未发现沉积物。在SLE患者中，LBT阳性与血清补体Clq结合活性（ClqBA）和低补体血症直接相关。平均ClqBA为13.49 ±。12.85和2.38 .+-。LBT阳性和阴性的SLE患者分别为2.27%（P < 0.005）。有皮肤沉积物者血清C3、C4水平明显低于无皮肤沉积物者（P < 0.005）。与正常血清相比，SLE患者的血清显示出溶解预先形成的免疫复合物的能力总体降低。10名LBT阳性患者溶解免疫复合物的能力低于来自LBT阴性狼疮患者的血清（45 . ±. 16和62 .+-. 11%; P < 0.01）。LBT阴性的SLE患者抑制免疫复合物沉淀的能力降低（P < 0.05）。因此，这些数据表明，在SLE患者中，补体介导的免疫复合物溶解的减少与高水平循环免疫聚集体的持续存在有关，考虑到其与阳性LBT的相关性，可能是皮肤DEJ处IG沉积的原因。
Immunofluorescent deposits at the dermal-epidermal junction (DEJ) of the skin (lupus band test or LBT) were evaluated in 134 patients with various connective tissue diseases. LBT was positive in 23 of 32 (71.8%) patients with systemic lupus erythematosus (SLE), in 3 of 53 (5.6%) patients with rheumatoid arthritis (RA), and in 1 of 5 cases of mixed connective tissue disease (MCTD). No deposits were found in patients with systemic sclerosis and with vasculitis. In patients with SLE a positive LBT showed a direct correlation with serum complement Clq binding activity (ClqBA) and with hypocomplementemia. The mean ClqBA was 13.49 .+-. 12.85 and 2.38 .+-. 2.27% in SLE patients with positive and negative LBT, respectively (P < 0.005). Likewise depressed mean serum levels of C3 and C4 were detected in patients with skin deposits (P < 0.005). Sera from SLE patients showed an overall decreased capacity to solubilize preformed immune complexes when compared to normal sera. Ten LBT-positive patients were less able to solubilize immune complexes than sera from LBT-negative lupus patients (45 .+-. 16 and 62 .+-. 11%, respectively; P < 0.01). Also the capacity to inhibit the precipitation of immune complexes was decreased in SLE patients with negative LBT (P < 0.05). Thus, these data suggest that in SLE patients a decreased complement-mediated solubilization of immune complexes is involved in the persistence of high levels of circulating immune aggregates and, considering its correlation with positive LBT, may be responsible for the deposits of Ig at the DEJ of the skin.