Distribution and Cytoarchitecture of Sympathetic Neurons Innervating the Pineal Gland in Chick: A CTB-HRP Study
Distribution and Cytoarchitecture of Sympathetic Neurons Innervating the Pineal Gland in Chick: A CTB-HRP Study
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鸡松果体交感神经元的分布和细胞结构：CTB-HRP 研究
DOI：
10.1111/j.1439-0264.2008.00901.x
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发表时间：
2009-02-01
影响因子：
0.9
通讯作者：
Chen, Y.
中科院分区：
文献类型：
--
作者：
Jia, L.;Wang, Z.;Chen, Y.
用霍乱毒素B亚单位-辣根过氧化物酶（CTB-HRP）逆行标记法，对鸡双侧上级颈神经节（SCG）内支配松果体的神经元进行标记。结果表明，这些交感神经元的胞体在颈上神经节内由吻侧向尾侧分布，主要集中在椎旁干的对侧。这些神经元的纤维收集的头颈动脉神经。根据投射到松果体的交感神经元的胞体面积和树突野的大小，这些交感神经元可分为四大类：I组细胞（52.4%），体细胞面积小（平均303.5 μ m（2））和狭窄的树枝状区域（平均3767.8 μ m（2）），II组细胞（39.0%）为中等大小的染色体区（473.3 μ m（2））和中型树枝状田（7522.2 μ m（2）），第III组细胞（6.4%）为中等大小的染色体区Ⅳ型细胞（2.2%）胞体面积大（940.7 μ m2），树突野宽（14553.2 μ m2）。在这些松果体投射神经元中，大多数呈现出较小的树突野。Ⅰ、Ⅱ组小、中型树突野神经元约占CTB-HRP标记神经元总数的91.4%，Ⅲ、Ⅳ组树突野较宽的神经元较少，仅占8.6%。
The neurons in bilateral superior cervical ganglia (SCG) innervating the chick pineal gland were labelled by using the technique of retrograde axonal labelling with cholera toxin B subunit linked to horseradish peroxidase (CTB-HRP). To our results, perikarya of these sympathetic neurons distributed from rostral to caudal in the SCG, and mainly localized in the opposite side of the paravertebral trunk. The fibres of these neurons were collected by the cephalic carotid nerve. According to the sizes of somal area and dendritic field, these sympathetic neurons projecting to the pineal gland were classified into four major groups: group I cells (52.4%) with a small somal area (303.5 mu m(2) on average) and narrow dendritic field (3767.8 mu m(2) on average), group II cells (39.0%) with a middle-sized somal area (473.3 mu m(2)) and middle-sized dendritic field (7522.2 mu m(2)), group III cells (6.4%) with a middle-sized somal area (473.4 mu m(2)) and wide dendritic field (13 104.4 mu m(2)), and group IV cells (2.2%) with a large somal area (940.7 mu m(2)) and wide dendritic field (14 553.2 mu m(2)). Of these pineal projecting neurons, most took on a lesser dendritic field. The neurons with small or middle-sized dendritic field from group I and II were about 91.4% of the total neurons labelled with CTB-HRP, and the neurons with wide dendritic field from group III and IV were less with 8.6%.