The recovery of 5-HT immunoreactivity in lumbosacral spinal cord and locomotor function after thoracic hemisection
The recovery of 5-HT immunoreactivity in lumbosacral spinal cord and locomotor function after thoracic hemisection
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DOI：
10.1006/exnr.1996.0094
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发表时间：
1996-06-01
影响因子：
5.3
通讯作者：
Perkins, R
中科院分区：
文献类型：
--
作者：
Saruhashi, Y;Young, W;Perkins, R
为了确定5-羟色胺（5-HT）在脊髓损伤后恢复中的作用，我们对68只大鼠进行了脊髓5-HT免疫组织化学检查，并评估了胸（T8）脊髓半横断后的运动恢复。48只大鼠行椎板切除加半切术，其余20只仅行椎板切除术。使用0-14点量表旷场试验，每隔一天评价所有大鼠直至4周。半切术显著降低了同侧和对侧的平均后肢评分，分别从14降低到1.5 +/- 0.32和5.6 +/- 0.31（平均值+/-平均值标准误）。4周后，所有大鼠均恢复了明显的正常行走。5-HT免疫组化研究显示，半切后1周，同侧腰骶髓中含5-HT的终末明显减少，但对侧腰骶髓中无。脊髓半切后4周，5-HT免疫反应阳性纤维和终末返回同侧腰骶髓，在胸水平有许多5-HT纤维穿过中央管。通过5-HT染色强度分级和5-HT终末计数来估计腰骶部腹角5-HT神经元的恢复。腰骶部腹角5-HT免疫反应性的恢复与运动恢复相关。运动恢复总是发生在5-HT终端的密度接近20%的控制值。这些结果表明，5-HT纤维和终末的返回预测胸脊髓半切后运动恢复的时间过程和程度。(C)出版社：Academic Press，Inc.
To determine the role of serotonin (5-HT) in recovery from spinal cord injury, we examined spinal cord 5-HT immunohistologically and assessed locomotor recovery after thoracic (T8) spinal cord hemisection in 68 rats. Forty eight rats had laminectomy and hemisection, while the remaining 20 rats received laminectomy only. All rats were evaluated every other day far 4 weeks, using a 0-14 point scale open field test. Hemisection markedly reduced mean hindlimbs scores from 14 to 1.5 +/- 0.32 and 5.6 +/- 0.31 (mean +/- standard error of mean) in the ipsilateral and contralateral side, respectively. The rats all recovered apparently normal walking by 4 weeks. The 5-HT immunohistological study revealed a marked reduction of 5-HT-containing terminals in the ipsilateral but not the contralateral lumbosacral cord by 1 week after hemisection. By 4 weeks after hemisection, 5-HT immunoreactive fibers and terminals returned to the ipsilateral lumbosacral cord, with many 5-HT fibers crossing over the central canal at thoracic level. We estimated the recovery of 5-HT neural elements in lumbosacral ventral horn by ranking 5-HT staining intensity and counting 5-HT terminals. The return of 5-HT immunoreactivity of the lumbosacral ventral horn correlated with locomotor recovery. Locomotory recovery invariably occurred when the density of 5-HT terminals approached 20% of control values. These results indicate that return of 5-HT fibers and terminals predict the time course and extent of locomotory recovery after thoracic spinal cord hemisection. (C) 1996 Academic Press, Inc.