On the mechanism of cytotoxicity of silver and copper amalgams in a cell culture system.
On the mechanism of cytotoxicity of silver and copper amalgams in a cell culture system.
复制标题
细胞培养系统中银和铜汞齐的细胞毒性机制。
DOI：
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发表时间：
1974
期刊：
影响因子：
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通讯作者：
J. Leirskar
中科院分区：
文献类型：
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作者：
J. Leirskar
– 银和铜汞合金对人上皮细胞单层培养物显示出明显的细胞毒性作用 (NCTG 2544)。对银汞合金培养物培养基的分析表明，锌被大量释放（孵育 24 小时后为 14 μg/ml）。少量的汞以及可能还有一些银被释放，而铜和锡则不会被释放。检测到。毒性试验表明，10μg Zn2+/ml孵育24小时后细胞数量减少96%。在铜汞合金文化中。 24 小时后，每毫升培养基中发现 100 μg 铜和 5 μg 镉。仅释放少量汞，毒性测试显示Cu2+和Cd2+的作用随着调节而增加。 50 μg Cu2+/ml 孵育 24 小时后，细胞数量减少 73%，3 天后，25 μg/ml 也有类似效果。使用 10 μg Cd2+/ml 24 小时后没有细胞残留，而 3 天后，1μg/ml 使细胞生长减少 80% 以上。
– Silver and copper amalgams show a pronounced cytotoxic effect on monolayer cultures of human epithelial cells (NCTG 2544). Analyses of the medium from silver amalgam cultures showed that, zinc was released in substantial amounts (14 μg/ml after incubation for 24 h). Small amounts of mercury and possibly also some silver were released, whereas release of copper and tin could not be. detected. Toxicity tests showed that 10 μg Zn2+/ml reduced the number of cells by 96% after incubation for 24 h. In the copper amalgam cultures about. 100 μg copper and 5 μg, cadmium per ml medium were found after 24 h. Only small amounts of mercury were released, Toxicity tests showed an increasing effect of Cu2+ and Cd2+ with tune. With 50 μg Cu2+/ml the number of cells was reduced by 73% after incubation for 24 h, and after 3 d a similar effect was found with 25 μg/ml. With 10 μg Cd2+/ml no cells were left after 24 h, whereas after 3 d 1μg/ml reduced cell growth by more than 80 %.