Constituents of Ocimum sanctum with antistress activity
Constituents of Ocimum sanctum with antistress activity
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DOI：
10.1021/np0700164
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发表时间：
2007-09-01
影响因子：
5.1
通讯作者：
Maurya, Rakesh
中科院分区：
文献类型：
--
作者：
Gupta, Prasoon;Yadav, Dinesh Kumar;Maurya, Rakesh
从圣罗勒（Ocimum sanctum）叶提取物中分离得到3个新化合物：芹菜素、芹菜素-7- o - β - d -葡萄糖醛酸、芹菜素-7- o - β - d -葡萄糖醛酸(4)、芹菜素-7- o - β - d -葡萄糖醛酸6”-甲酯、木犀草素-7- o - β - d -葡萄糖醛酸6”-甲酯、木犀草素-7- o - β - d -葡萄糖醛酸、木犀草素-5- o - β - d -葡萄糖醛酸、和4-烯丙基-1- o - β - d -葡萄糖吡诺基-2-羟基苯(5)，以及两种已知的脑苷。新化合物的结构是在广泛的一维和二维核磁共振光谱分析的基础上确定的。新化合物（1-3）和已知化合物4、5在40 mg/kg体重剂量下对雄性Sprague-Dawley大鼠急性应激诱导的生化变化进行了筛选。化合物1通过使高血糖、血浆皮质酮、血浆肌酸激酶和肾上腺肥大正常化显示出良好的抗应激作用。化合物2和5也能有效地正化这些应力参数。相比之下，化合物3和4在使这些效果正常化方面无效。
Three new compounds, ocimumosides A (1) and B (2) and ocimarin (3), were isolated from an extract of the leaves of holy basil (Ocimum sanctum), together with eight known substances, apigenin, apigenin-7-O-beta-D-glucopyranoside, apigenin-7-O-beta-D-glucuronic acid (4), apigenin-7-O-beta-D-glucuronic acid 6 ''-methyl ester, luteolin-7-O-beta-D-glucuronic acid 6 ''-methyl ester, luteolin-7-O-beta-D-glucopyranoside, luteolin-5-O-beta-D-glucopyranoside, and 4-allyl-1-O-beta-D-glucopyronosyl-2-hydroxybenzene (5), and two known cerebrosides. The structures of the new compounds were determined on the basis of extensive 1D and 2D NMR spectroscopic analysis. The new compounds (1-3) and the known compounds 4 and 5 were screened at a dose of 40 mg/kg body weight for acute stress-induced biochemical changes in male Sprague-Dawley rats. Compound 1 displayed promising antistress effects by normalizing hyperglycemia, plasma corticosterone, plasma creatine kinase, and adrenal hypertrophy. Compounds 2 and 5 were also effective in normalizing most of these stress parameters. In contrast, compounds 3 and 4 were ineffective in normalizing any of these effects.