Endocrine regulation of the secretion of mucin in the tubal epithelium of the rabbit
Endocrine regulation of the secretion of mucin in the tubal epithelium of the rabbit
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兔输卵管上皮粘蛋白分泌的内分泌调节
DOI：
10.1002/ar.1091300302
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发表时间：
1958
期刊：
影响因子：
--
通讯作者：
G. S. Greenwald
中科院分区：
文献类型：
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作者：
G. S. Greenwald
在交配后的前三天内，每天注射5 μ g 17-β-雌二醇苯甲酸酯可中断家兔妊娠。这种处理减少了卵在输卵管中时沉积在卵周围的粘蛋白的量，因此，据推测，缺乏粘蛋白是囊胚进入子宫后不久死亡的原因（Greenwald，'57）。包裹卵子的这一层长期以来一直被称为“蛋白层”，但最近发现它主要由粘多糖组成（Braden，'52 ; Bacsich和汉密尔顿，'54）。其他研究者已经描述了输卵管上皮分泌这一层（Moreaux，'13; Cohnen，'27; Westman，'30），但还没有人确定激素在调节这种活动中的作用。因此，作者从三个方面着手：第一，注射最有效的天然雌激素第二，通过尝试将调节粘蛋白合成的激素与那些与粘蛋白从细胞中释放有关的激素分开（这是通过将这些激素与雌激素（雌二醇））注射到兔子体内，并将这些动物的输卵管上皮与对照组的输卵管上皮进行比较（在先前的实验中，注意力集中在卵子上）;第二，通过尝试将调节粘蛋白合成的激素与那些与粘蛋白从细胞中释放有关的激素分开（这是通过将雌激素与雌激素分开来实现的），
Pregnancy in the rabbit can be interrupted by daily injection of 5 pg of 17-P-estradiol benzoate during the first three days after coitus. Such treatment reduces the amount of mucin deposited around the ova while they are in the tubes and so, presumably, the lack of mucin is the factor responsible for the death of the blastocysts soon after they enter the uterus (Greenwald, ’57). This layer which invests the ova has long been referred to as the “albumen layer,” but recently it has been shown to consist primarily of a mucopolysaccharide (Braden, ’52 ; Bacsich and Hamilton, ’54). Other investigators have described the secretion of this layer by the tubal epithelium (Moreaux, ’13; Cohnen, ’27; Westman, ’30) but no one has yet determined the role of hormones in regulating this activity. Accordingly, the writer has attacked the problem in three ways: first, by injecting the most potent natural estrogen (estradiol) into rabbits and comparing the tubal epithelium of these animals with that of controls (in preceding experiments, attention had been centered on the ova) ; second, by attempting to separate the hormones regulating the synthesis of mucin from those concerned with its release from the cell (this was accomplished