Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination
Manipulation of the 1RS.1BL translocation in wheat by induced homoeologous recombination
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DOI：
10.2135/cropsci2000.401216x
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发表时间：
2000-01-01
期刊：
影响因子：
2.3
通讯作者：
Lukaszewski, AJ
中科院分区：
文献类型：
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作者：
Lukaszewski, AJ
黑麦（Secale cereale L.）1 R染色体在小麦（Triticum aestivum L.）育种，因为它们赋予对几种病虫害的抗性并提高产量。它们的主要缺点是降低了面包的制作质量。为了弥补这一缺陷，黑麦染色体臂IRS易位1RS.1BL和1RS.1DL被诱导的ph 1b突变与小麦组1染色体的短臂重组。在20234个子代中，共回收重组染色体139条，其中IBS型103条，1AS型22条，1DS型14条。小麦的Gli-1/Glu-3基因座与黑麦的Sec-1基因座为非同源等位基因，在黑麦染色体上被一个约13厘米长的片段隔开，该片段含有抗病基因座Pm 8、Lr 26、Sr 31和Yr 9。一组超过30条染色体，其中Sec-1莲花被不同长度的1BS片段取代，以及两条染色体，其中1BS的1.4-和3.2-cM片段引入Gli-1/Glu-3位点。从每个类别中选择的染色体被允许在分离插入小麦片段的1 RS的共享片段内重组，并回收两个三级重组染色体。在细胞学上，这些染色体表现为正常的1 RS臂，但每个都有两个1BS的插入片段：一个引入Gli-1/Glu-3位点，第二个去除Sec-1位点。由于所获得的易位系的蛋白质组成与正常小麦的蛋白质组成相同，因此认为这些操作可以消除与1 RS. 1BL易位相关的品质缺陷。
Centric translocations of the short arm Of rye (Secale cereale L.) chromosome 1R are useful in wheat (Triticum aestivum L.) breeding because they confer resistance to several pests and diseases and improve yield. Their major disadvantage is in reduced bread making quality. To remedy this defect, rye chromosome arm IRS in translocations 1RS.1BL and 1RS.1DL was induced by the ph1b mutation to recombine with the short arms of wheat group-1 chromosomes. Among 20 234 progeny screened, 139 primary recombinant chromosomes were recovered including 103 with IBS, 22 with 1AS and 14 with 1DS. The Gli-1/Glu-3 loci of wheat were non-homoeoallelic to the Sec-1 locus of rye and were separated by about a 13-cM-long segment, which on the rye chromosome contained disease resistance loci Pm8, Lr26, Sr31, and Yr9, Pairs of primary recombinants 1RS-1BS with breakpoints flanking the storage protein loci were intercrossed and two types of secondary recombinant chromosomes 1RS were produced: a group of over 30 chromosomes where the Sec-1 lotus was replaced bg segments of 1BS of various lengths, and two chromosomes where 1.4- and 3.2-cM segments of 1BS introduced the Gli-1/Glu-3 loci. Selected chromosomes from each class vc ere allowed to recombine within the shared segments of 1RS separating the intercalary wheat segments and two tertiary recombinant chromosomes were recovered. Cytologically, these chromosomes appear as normal 1RS arms but each has two intercalary segments of 1BS: one introducing the Gli-1/Glu-3 loci and the second one removing the Sec-1 locus. Because the protein cpmposition of the resulting translocation lines is identical to that of normal wheat, it is believed that these manipulations could eliminate the quality defect associated with the 1RS.1BL translocation.