Reactive oxygen species and nitric oxide are involved in ABA inhibition of stomatal opening.
Reactive oxygen species and nitric oxide are involved in ABA inhibition of stomatal opening.
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DOI：
10.1111/j.1365-3040.2007.01711.x
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发表时间：
2007-10
期刊：
影响因子：
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通讯作者：
Jiupiang Yan;Nobue Tsuichihara;T. Etoh;S. Iwai
中科院分区：
文献类型：
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作者：
Jiupiang Yan;Nobue Tsuichihara;T. Etoh;S. Iwai
虽然一氧化氮（NO）和活性氧（ROS）是介导脱落酸（ABA）诱导的气孔关闭所必需的信号分子，但这些分子是否也介导ABA对气孔打开的抑制尚不清楚。在本研究中，我们研究了NO和ROS在ABA抑制蚕豆气孔开放中的作用。ABA诱导NO和ROS合成，NO清除剂降低了ABA对气孔开启的抑制作用。外源NO和过氧化氢（H2O2）也能抑制气孔打开，表明NO和ROS参与了抑制信号传导过程。一氧化氮合酶（NOS）抑制剂逆转了ABA对气孔开放的抑制。NO清除剂和NOS抑制剂也逆转了H2O2对气孔张开的抑制过程。我们发现，在ABA抑制气孔打开过程中，NO在ROS的信号传导过程中处于下游，并且由一种类似nos的酶合成。
Although nitric oxide (NO) and reactive oxygen species (ROS) are essential signalling molecules required for mediation of abscisic acid (ABA)-induced stomatal closure, it is not known whether these molecules also mediate the ABA inhibition of stomatal opening. In this study, we investigated the role of NO and ROS in the ABA inhibition of stomatal opening in Vicia faba. ABA induced both NO and ROS synthesis, and the NO scavenger reduced the ABA inhibition of stomatal opening. Exogenous NO and hydrogen peroxide (H2O2) also inhibited stomatal opening, indicating that NO and ROS are involved in the inhibition signalling process. An inhibitor of nitric oxide synthase (NOS) reversed the ABA inhibition of stomatal opening. Either the NO scavenger or the NOS inhibitor also reversed the process in the H2O2 inhibition of stomatal opening. We found that in the ABA inhibition of stomatal opening, NO is downstream of ROS in the signalling process, and NO is synthesized by a NOS-like enzyme.