Immunological development of preterm infants in early infancy
Immunological development of preterm infants in early infancy
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DOI：
10.1111/j.1365-2249.2005.02741.x
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发表时间：
2005-04
影响因子：
4.6
通讯作者：
B. Zhang;Y. Ohtsuka;T. Fujii;H. Baba;K. Okada;H. Shoji;S. Nagata;Tomoaki Shimizu;Y. Yamashiro-Y.-Yamas
中科院分区：
文献类型：
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作者：
B. Zhang;Y. Ohtsuka;T. Fujii;H. Baba;K. Okada;H. Shoji;S. Nagata;Tomoaki Shimizu;Y. Yamashiro-Y.-Yamas
为评价早产儿尤其是早期婴儿的免疫发育状况，我们检测了16例孕37（33.63 ± 3.29）周出生的低出生体重儿（1720.38 ± 502.80 g）在出生后0、14和28 d血清细胞因子水平和Th 2和Th 1趋化因子受体CCR 4和CCR 5的表达。采用酶联免疫吸附试验（ELISA），血清白细胞介素（IL）-4水平在第14天升高，但在第28天降至初始水平（P < 0·05）。血清转化生长因子（TGF）-β水平在第14天显著升高（P <0.05），但在第28天降至初始水平（P <0.05）。采用逆转录-聚合酶链反应（RT-PCR）和流式细胞术分析CCR 4和CCR 5的表达。RT-PCR证实出生后不久CCR 5-mRNA表达，而CCR 4-mRNA无表达。此后，CCR 4-mRNA表达显著增加，并在28天达到CCR 5-mRNA表达水平（P < 0·05）。然而，流式细胞术分析显示，CCR 4和CCR 5的表达水平在出生时都很低。CCR 4 + CD 4+细胞在0-28天显著增加（P <0.05），而CCR 5 + CD 4+细胞在0- 28天无显著变化。IL-4和TGF-β合成的增加以及CCR 4 + CD 4+细胞的增加表明，在母体外的情况下，即使在分娩后不久的早产儿中，Th 2反应也会发生偏移，而他们可能能够在出生后不久发展Th 1介导的反应。
To evaluate the immunological development of preterm infants, especially in early infancy, we examined the serum cytokine levels and the expression of Th2 and Th1 chemokine receptors, CCR4 and CCR5, on days 0, 14 and 28 in 16 low birth weight infants (1720·38 ± 502·80 g) born at less than 37 (33·63 ± 3·29) weeks of gestation. Using an enzyme‐linked immunosorbent assay (ELISA), serum interleukin (IL)‐4 levels exhibited an increase on day 14, but decreased to the initial level on day 28 (P < 0·05). The significant elevation of serum transforming growth factor (TGF)‐β levels was confirmed on day 14 (P < 0·05) but decreased to the initial level on day 28 (P < 0·05). The expression of CCR4 and CCR5 were examined using reverse transcription‐polymerase chain reaction (RT‐PCR) and flow cytometric analysis. The RT‐PCR confirmed the expression of CCR5‐mRNA soon after birth, while there was no expression of CCR4‐mRNA. Thereafter, the expression of CCR4‐mRNA increased significantly and reached the level of CCR5‐mRNA expression on day 28 (P < 0·05). Flow cytometric analysis, however, revealed that the expression levels of both CCR4 and CCR5 were low at birth. Thus, CCR4+ CD4+ cells were significantly increased from days 0–28 (P < 0·05), while CCR5+ CD4+ cells were not. Increased IL‐4 and TGF‐β synthesis as well as increased CCR4+ CD4+ cells suggest that, under extra‐maternal circumstances, there is a shift in bias toward Th2 responses even in preterm infants soon after delivery, while they may be capable of developing Th1 mediated responses soon after birth.