Prevalence, types, and RNA concentrations of human parechoviruses, including a sixth parechovirus type, in stool samples from patients with acute enteritis
Prevalence, types, and RNA concentrations of human parechoviruses, including a sixth parechovirus type, in stool samples from patients with acute enteritis
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DOI：
10.1128/jcm.01468-07
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发表时间：
2008-01-01
影响因子：
9.4
通讯作者：
Drosten, Christian
中科院分区：
文献类型：
--
作者：
Baumgarte, Sigrid;Luna, Luciano Kleber de Souza;Drosten, Christian
副孤病毒流行病学和疾病关联尚不完全清楚。对两组患者的粪便样本进行了所有人类副肠孤病毒 (HPeV) 的实时逆转录酶 PCR (RT-PCR) 检测。两组均包含所有年龄段的急性肠炎患者，观察时间为一整年。诺如病毒、腺病毒、肠道病毒、星状病毒或轮状病毒感染的患者被排除在外。 118名患者来自暴发和。医院环境中，未检测到 HPeV。在一项由 499 名非住院患者组成的前瞻性研究组中，检出率为 1.6%。从 39 名对照患者中检测出 1 名病毒阳性患者。阳性样本仅出现在夏季和秋季。只有一名患者出现伴随的呼吸道症状。未发现与旅行或动物接触有关。除一名患者外，所有阳性患者均小于 2 岁，性别比例为中性。在<2岁儿童中，检出率为11.6%（60名儿童中有7名）。每克或每毫升粪便的病毒载量范围为 3,170 至 503,377,290 拷贝。最高病毒载量之一发生在测试时没有症状的对照儿童中。系统发育分析显示，我们的患者中主要存在当代 HPeV1 毒株，但也显示出 HPeV1 与历史原型毒株的进化转变中的一个单独的新谱系。此外，还确定了一种新的第六种 HPeV 类型。对两种病毒的全基因组分析显示，一种病毒中存在 HPeV1 和 -3 之间的重组，另一种病毒中存在 HPeV6 和 -1 之间的重组。 HPeV 似乎与 2 岁以下儿童相关，并且针对 HPeV 的特异性 RT-PCR 应纳入肠炎筛查中。
Parechovirus epidemiology and disease association are not fully understood. Real-time reverse transcriptase PCR (RT-PCR) for all human parechoviruses (HPeV) was applied on stool samples from two groups of patients. Both groups contained patients with acute enteritis of all age groups, seen during one full year. Patients with norovirus, adenovirus, enterovirus, astrovirus, or rotavirus infections were excluded. In 118 patients from outbreak and. hospital settings, no HPeV was detected. In a prospective study group of 499 nonhospitalized patients, the detection rate was 1.6%. One virus-positive patient was detected from 39 control patients. Positive samples occurred only in summer and autumn. Only one patient had accompanying respiratory symptoms. An association with travel or animal contact was not found. All positive patients except one were < 2 years of age, with a neutral gender ratio. In children < 2 years of age, the detection rate was 11.6% (7 of 60 children). The range of viral loads was 3,170 to 503,377,290 copies per gram or milliliter of stool. One of the highest viral loads occurred in a control child without symptoms at the time of testing. Phylogenetic analysis showed mainly contemporary HPeV1 strains in our patients but also showed a separate new lineage of HPeV1 in evolutionary transition from the historical prototype strain. Moreover, a novel sixth HPeV type was identified. Full genome analysis of the two viruses revealed recombination between HPeV1 and -3 in one and HPeV6 and -1 in another. HPeV seems relevant in children < 2 years and specific RT-PCR for HPeV should be included in enteritis screening.