Transcriptional Insights into the Sugarcane-Sorghum mosaic virus Interaction
Transcriptional Insights into the Sugarcane-Sorghum mosaic virus Interaction
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甘蔗-高粱花叶病毒相互作用的转录见解
DOI：
10.1007/s12042-018-9210-6
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发表时间：
2018-12-01
影响因子：
2
通讯作者：
Xu, Liping
中科院分区：
文献类型：
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作者：
Ling, Hui;Huang, Ning;Xu, Liping
花叶病是一种主要的病毒性疾病，严重影响糖含量和甘蔗生产。在花叶病病原体中，高粱花叶病毒（SrMV）是最流行的一种。为了更好地了解甘蔗与SrMV之间的相互作用机制，对甘蔗品种ROC22感染SrMV和脱毒的叶片样本进行了深度测序，并通过qRT-PCR验证了测序数据。总共获得了89,338个unigenes、481个差异表达unigenes和51个Potyvirus宿主相互作用基因（PHI）同源序列。 RNA-seq数据表明，SrMV从内质网（ER）到叶绿体复制的增加导致叶绿体损伤，随后Ca2+、ROS、细胞分裂素、生长素和乙烯信号传导相关基因的差异表达开始，以及一些防御相关基因的转录。在 51 个 PHI 中，两个 SrMV 抗性和两个 SrMV 易感甘蔗品种在 SrMV 感染下钙调蛋白相关蛋白基因和乙烯诱导转录因子基因上调，表明这两个基因可以作为潜在的基因敲除靶点来创建 SrMV 不相容种质。此外，基于SrMV抗感甘蔗品种中的反向表达模式，编码热休克蛋白70、叶绿体rieske Fe/S蛋白、网织蛋白同源结构域蛋白和水杨酸结合蛋白3的基因可作为鉴定甘蔗材料对SrMV抗性或敏感性的潜在标记。这项研究应有助于了解甘蔗品种抗 SrMV 的分子机制。
Mosaic disease is a major viral disease that severely compromises sugar content and cane production. Among mosaic disease pathogens, Sorghum mosaic virus (SrMV) is the most prevalent one. To better understand the interaction mechanism between sugarcane and SrMV, samples of SrMV-infected and virus-free leaves of sugarcane cultivar ROC22 were deep sequenced and the sequencing data was confirmed by qRT-PCR. In total, 89,338 unigenes, 481 differentially expressed unigenes and 51 homologous sequences of Potyvirus host interactor (PHI) genes were obtained. The RNA-seq data implied that, the increase of SrMV replication from endoplasmic reticulum (ER) to chloroplast led to chloroplasts damage, following the initiation of differential expression of genes related to Ca2 +, ROS, cytokinin, auxin, and ethylene signaling, and also the transcription of some defense related genes. Among 51 PHIs, the upregulation of a calmodulin-related protein gene and an ethylene-inducible transcription factor gene in two SrMV-resistant and two SrMV-susceptible sugarcane cultivars under SrMV infection suggests that these two genes could be used as potential gene knockout targets for creating SrMV incompatible germplasm. Besides, based on its inverse expression pattern in the SrMV-resistant and -susceptible sugarcane cultivars, genes encoding heat shock protein 70, chloroplastic rieske Fe/S protein, reticulon homology domain protein and salicylic acid binding protein 3 might be used as the potential markers for identifying the resistance or susceptibility of sugarcane materials to SrMV. This study should help to understand the molecular mechanisms underlying SrMV-resistance of sugarcane cultivars.