Biologic and immunologic characteristics of cold-adapted influenza virus.
Biologic and immunologic characteristics of cold-adapted influenza virus.
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DOI：
10.4049/jimmunol.102.3.728
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发表时间：
1969-03
影响因子：
4.4
通讯作者：
H. F. Maassab
中科院分区：
文献类型：
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作者：
H. F. Maassab
流感病毒的冷变异体在酸性pH值（5.7至6.3）和高温（41°C）下的繁殖能力受损。这些特征被用作区分冷变异与原始“野生”菌株的标记。在小鼠中连续传代8次后，从感染小鼠肺部恢复的病毒株仍然可以通过这些选择性标记识别为感冒变体。在小鼠中连续十次传代后，它们的毒力没有恢复，并保留了引发良好抗体反应的能力。雪貂对感冒变种感染的反应与小鼠一样；这些动物没有显示出疾病的迹象，并产生了良好的HI抗体反应。此外，具有循环抗体的雪貂在鼻内再次接种感冒变异病毒后显示出更高的抗体反应。
Cold variants of influenza virus showed an impaired capacity to reproduce at acid pH (5.7 to 6.3) and elevated temperature (41°C). These features were used as markers to differentiate the cold variant from the original “wild” strain. After eight consecutive passages in mice, the viral strains recovered from the lungs of infected mice could still be identified by these selective markers as the cold variants. They showed no reversion to virulence upon ten consecutive passages in mice and retained their capacity to elicit a good antibody response. Ferrets responded as did mice to infection with the cold variants; the animals showed no evidence of disease and developed good HI antibody response. In addition, ferrets that possessed circulating antibody showed a heightened antibody response after they were reinoculated intranasally with cold variant virus.