Evidence of HLA-DR antigen biosynthesis by human keratinocytes in disease.
Evidence of HLA-DR antigen biosynthesis by human keratinocytes in disease.
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人类角质形成细胞在疾病中生物合成 HLA-DR 抗原的证据。
DOI：
10.1084/jem.159.6.1784
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发表时间：
1984-06-01
期刊：
影响因子：
--
通讯作者：
Stingl G
中科院分区：
文献类型：
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作者：
Volc-Platzer B;Majdic O;Knapp W;Wolff K;Hinterberger W;Lechner K;Stingl G
与HLA-DR表达仅限于朗格汉斯细胞（LC）群体的正常人皮肤相反，在某些疾病状态下，HLA-DR抗原而不是HLA-DS抗原可以在角质形成细胞（KC）上容易地检测到，即，皮肤T细胞淋巴瘤（CTCL）、移植物抗宿主病（GVHD）和扁平苔藓（LP）。为了阐明KC结合的HLA-DR抗原的细胞起源，我们使用了针对仅在细胞质HLA-DR γ链（VIC-Y1）上表达的决定簇的单克隆抗体，并通过免疫荧光观察到，在其表面上展示HLA-DR α/β复合物的KC均匀地显示细胞质VIC-Y1反应性。鉴于γ链对HLA-DR生物合成的重要作用，我们得出结论，KC上的HLA-DR抗原是由这些细胞主动合成的。
As opposed to normal human skin where HLA-DR expression is restricted to the Langerhans cell (LC) population, HLA-DR, but not HLA-DS antigens can be readily detected on keratinocytes (KC) in certain disease states, i.e., cutaneous T cell lymphoma (CTCL), graft-vs-host disease (GVHD), and lichen planus (LP). To clarify the cellular origin of KC- bound HLA-DR antigens, we used a monoclonal antibody directed against determinants solely expressed on the cytoplasmic HLA-DR gamma chain (VIC-Y1) and observed that, by immunofluorescence, KC displaying HLA-DR alpha/beta complexes on their surface uniformly displayed cytoplasmic VIC-Y1 reactivity. In view of the crucial role of the gamma chain for HLA-DR biosynthesis, we conclude that HLA-DR antigens on KC are actively synthesized by these cells.