DIETARY RESTRICTION AND AGING - THE INITIATION OF A PRIMATE STUDY
DIETARY RESTRICTION AND AGING - THE INITIATION OF A PRIMATE STUDY
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DOI：
10.1093/geronj/45.5.b148
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发表时间：
1990-09-01
期刊：
影响因子：
--
通讯作者：
ROTH, GS
中科院分区：
文献类型：
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作者：
INGRAM, DK;CUTLER, RG;ROTH, GS
幼年（1岁）和成年（3-5岁）雄性恒河猴（Macaca Mulatta）和幼年（1-4岁）和成年（5-10岁）雄性松鼠猴（Saimiri Sciureus）根据年龄和体重推荐的热量摄入量，以自由采食或接近自由采食的水平饲喂饲料，或在3个月内逐渐引入这种限制，饲喂相同饲料的30%。从研究第一年开始，对这些对照组和实验组的体重进行分析，结果表明，与对照值相比，进行饮食限制的猴的体重增加速度明显较慢。饮食限制组的实际食物摄入量比对照组减少了22-24%。在研究的第一年，对血液学和血液化学测量的定期分析发现，控制组和实验组之间几乎没有显着差异，表明饮食限制对一般健康无害。当将幼龄和成年组（对照组和实验组合并）的血液学和血液化学测量值与每个物种的自由采食老年猴（恒河猴> 18岁;松鼠猴> 10岁）进行比较时，注意到许多显著的年龄差异。两个种属中最大和最一致的发现是淋巴细胞浓度、血清谷氨酸-乙酸转氨酶、血清谷氨酸-乙酸转氨酶、碱性磷酸酶和磷酸盐以及白蛋白/球蛋白比和血尿素氮-肌酐比的年龄相关性降低。这些参数以及许多其他在研究中实施的参数将被进一步监测，以确定饮食限制是否会影响发育速度以及衰老，正如在许多啮齿动物研究中观察到的那样。
Juvenile (1 yr) and adult (3-5 yr) male rhesus monkeys (Macaca Mulatta) and juvenile (1-4 yr) and adult (5-10 yr) male squirrel monkeys (Saimiri Sciureus) were fed a diet at or near ad libitum levels based on recommended caloric intake for age and body weight or fed 30% less of the same diet with this restriction gradually introduced over a 3-mo period. Analysis of body weights among these respective control and experimental groups from the first year of the study indicated that the monkeys undergoing dietary restriction were gaining weight at a markedly slower rate compared to control values. Actual food intake among diet-restricted groups had been reduced 22-24% below control levels. Periodic analysis ofhematology and blood chemistry measurements over the first year of the study detected few significant differences between control and experimental groups to indicate that diet restriction was not detrimental to general health. When values obtained from hematology and blood chemistry measurements of juvenile and adult groups (control and experimental groups combined) were compared to ad libitum fed old monkeys from each species (> 18 yr for rhesus; > 10 yr for squirrel monkeys), many significant age differences were noted. Among the largest and most consistent findings in both species were age-related decreases in concentrations of lymphocytes, serum glutamic oxalacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, and phosphates as well as the albumin/globulin ratio and the blood urea nitrogenlcreatinine ratio. Age-related increases in serum globulin and creatinine concentrations were also found. These parameters as well as many others being implemented in the study will be monitored further to determine if diet restriction affects the rate of development as well as aging as observed in numerous rodent studies applying such nutritional manipulations.