A placental sub-proteome: The apical plasma membrane of the syncytiotrophoblast
A placental sub-proteome: The apical plasma membrane of the syncytiotrophoblast
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DOI：
10.1016/j.placenta.2011.12.010
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发表时间：
2012-03-01
期刊：
影响因子：
3.8
通讯作者：
Robinson, J. M.
中科院分区：
文献类型：
--
作者：
Vandre, D. D.;Ackerman, W. E.;Robinson, J. M.
胎盘作为一种高度血管化的组织，在母体和胎儿循环之间介导气体和溶质交换。在人类胎盘中，与母体血液接触的界面是一种独特的上皮结构，称为合体滋养层。以前，我们开发了一种胶体二氧化硅为基础的方法，以产生高度富集的制剂的顶端质膜的合体滋养层。使用类似的制剂，这一重要的亚蛋白质组的蛋白质组学评估已确定340蛋白质的一部分，这顶膜馏分。其中38种蛋白质在人胎盘合体滋养层中的表达是未知的。与以前的研究一起，目前的蛋白质组数据库扩展了我们对正常胎盘合体滋养层顶端质膜蛋白质组的了解，包括500多种蛋白质。这个数据库是一个宝贵的资源，为未来的比较，以患病胎盘。此外，该数据集为进一步的胎盘和滋养层功能的实验研究提供了基础。(C)2011爱思唯尔有限公司保留所有权利。
As a highly vascularized tissue, the placenta mediates gas and solute exchange between maternal and fetal circulations. In the human placenta, the interface with maternal blood is a unique epithelial structure known as the syncytiotrophoblast. Previously we developed a colloidal-silica based method to generate highly enriched preparations of the apical plasma membrane of the syncytiotrophoblast. Using similar preparations, a proteomics assessment of this important sub-proteome has identified 340 proteins as part of this apical membrane fraction. The expression of 38 of these proteins was previously unknown in the human placental syncytiotrophoblast. Together with previous studies, the current proteomic database expands our knowledge of the proteome of the syncytiotrophoblast apical plasma membrane from normal placentas to include more than 500 proteins. This database is a valuable resource for future comparisons to diseased placentas. Additionally, this data set provides a basis for further experimental studies of placenta and trophoblast function. (C) 2011 Elsevier Ltd. All rights reserved.