Molecularly imprinted solid phase extraction coupled to high performance liquid chromatography for determination of aflatoxin M1 and B1 in foods and feeds
Molecularly imprinted solid phase extraction coupled to high performance liquid chromatography for determination of aflatoxin M1 and B1 in foods and feeds
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DOI：
10.1039/c4ra16784h
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发表时间：
2015-02
期刊：
影响因子：
3.9
通讯作者：
Shoulian Wei;Yong Liu;Zijun Yan;Ling Liu
中科院分区：
文献类型：
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作者：
Shoulian Wei;Yong Liu;Zijun Yan;Ling Liu
以黄曲霉毒素B1为模板分子，甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯为交联剂，Span 80和十六烷基三甲基溴化铵为表面活性剂，正十六烷为疏水剂，采用细乳液聚合法合成了黄曲霉毒素B1分子印迹聚合物。通过红外光谱、核磁共振氢谱、扫描电镜、激光散射和吸附实验对印迹聚合物进行了表征，结果表明印迹聚合物对黄曲霉毒素B1和M1具有良好的识别和选择性。以制备的聚合物为固相萃取吸附剂，建立了一种高选择性的样品前处理-高效液相色谱-荧光检测方法，用于食品和饲料中黄曲霉毒素B1和M1的测定。该方法对黄曲霉毒素M1和B1的检测限和定量限分别为0.05 μg kg−1和0.16 μg kg−1。大麦、花生油、饲料和啤酒加标样品的平均回收率为83%~ 96%。这些样品的精密度范围为2.2%至5.6%。作为一种预处理技术，所提出的方法比法规2006/40/EC更有效、更经济。
A molecularly imprinted polymer was synthesized by a miniemulsion polymerization method using aflatoxin B1 as the molecular template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate as the cross-linker, Span80 and hexadecyl trimethyl ammonium bromide as the surfactants and n-hexadecane as the hydrophobic reagent in the presence of water. This imprinted polymer was characterized by FT-IR, 1H NMR, scanning electron microscopy, laser light scattering and adsorption experiments, in which the results showed good recognition and selectivity to aflatoxin B1 and M1. Using the prepared polymer as a solid phase extraction sorbent, a highly selective sample pre-treatment method combined with high performance liquid chromatography and fluorescence detection was developed for determination of aflatoxin B1 and M1 in foods and feeds. The limit of detection and limit of quantification of this method for aflatoxins M1 and B1 were 0.05 μg kg−1 and 0.16 μg kg−1, respectively. The average recovery values from barley, peanut oil, feed and beer spiked samples ranged from 83% to 96%. The precision ranged from 2.2% to 5.6% for these samples. The proposed method was found to be more effective and economical as a pre-treatment technique than regulation 2006/40/EC.