Fine specificity of serum anticollagen molecules in experimental immune synovitis.
Fine specificity of serum anticollagen molecules in experimental immune synovitis.
复制标题
实验性免疫滑膜炎中血清抗胶原分子的精细特异性。
DOI：
10.1136/ard.44.5.328
复制
发表时间：
1985
影响因子：
27.4
通讯作者：
Moskowitz,RW
中科院分区：
文献类型：
--
作者：
Kresina,TF;Rosner,IA;Goldberg,VM;Moskowitz,RW
采用固相放射免疫分析法（RIA）测定兔igg诱导的免疫性滑膜炎模型血清中天然胶原和变性间质胶原的抗胶原抗体水平。在100%的实验动物（n = 6）中观察到结合天然间质胶原和变性II型胶原的血清抗体。在83%的动物中观察到α 1 （III）胶原多肽链的抗体，而在33%的动物中观察到变性I型胶原的血清抗体。抑制研究表明，血清抗胶原抗体具有构象依赖性和胶原类型特异性。此外，这些抗体群体对特定底物的亲和力变化高达2.81倍。滑膜炎抗胶原抗体结合天然II型胶原的平均结合常数（Ka）的平均值为5.47 X 10(6)mol；结合变性III型胶原的滑膜炎抗体的Ka为1.94 × 10(6)/mol。这些数据表明，在实验性igg诱导的慢性免疫性滑膜炎动物血清中表达了独特的抗胶原抗体群。
Serum anticollagen antibodies to the native and denatured interstitial collagens were measured by solid phase radioimmunoassay (RIA) in a rabbit model of IgG-induced immune synovitis. Serum antibodies binding the native interstitial collagens and denatured type II collagen were observed in 100% of the animals tested (n = 6). Titerable antibodies to the alpha 1 (III) collagen polypeptide chain were observed in 83% of the animals, whereas serum antibodies to denatured type I collagen were observed in 33%. Inhibition studies showed that the observed serum anticollagen antibodies were conformationally dependent and collagen type specific. In addition these antibody populations varied in their affinities by as much as a factor of 2.81 for the specific substrates. Mean value of the average binding constants (Ka) for synovitis anticollagen antibodies binding native type II collagen was 5.47 X 10(6)mol; while the Ka determined for synovitis antibodies binding denatured type III collagen was 1.94 X 10(6)/mol. The data indicate that unique anticollagen antibody populations are expressed in the serum of animals with experimental IgG-induced chronic immune synovitis.