Capsular Polysaccharide Types and Virulence-Related Traits of Epidemic KPC-Producing Klebsiella pneumoniae Isolates in a Chinese University Hospital
Capsular Polysaccharide Types and Virulence-Related Traits of Epidemic KPC-Producing Klebsiella pneumoniae Isolates in a Chinese University Hospital
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中国某大学医院流行产 KPC 肺炎克雷伯菌的荚膜多糖类型和毒力相关特征
DOI：
10.1089/mdr.2016.0222
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发表时间：
2017-10-01
影响因子：
2.6
通讯作者：
Zhang, Wei
中科院分区：
文献类型：
--
作者：
Liu, Yang;Liu, Pan-pan;Zhang, Wei
肺炎克雷伯氏菌是一种重要的人类病原菌，与多种疾病有关。肺炎克雷伯氏菌（KPC-Kp）迅速增加。荚膜是K.肺炎。在这项研究中，我们决定首先系统地表征KPC-Kp菌株中的荚膜多糖（CPS）和毒力性状。从中国某医院的临床标本中回收了56株KPC-Kp分离株，通过多位点序列分型（MLST）将其分配到克隆谱系。通过分子方法对荚膜分型（wzi测序和wzc聚合酶链反应[PCR]）和毒力基因进行表征。这些菌株的毒力是由生物膜形成，血清杀伤阻力，吞噬作用，和感染模型。56株KPC-Kp分离株中有6株不同的ST，其中ST 11型占76.8%（43/56），ST 147型6株，ST 15型4株，ST 1456型1株，ST 65型1株，ST 23型1株。根据wzi基因的DNA序列和wzc PCR，将这56株菌株分为荚膜型wzi 47-K47（n = 37）、wzi 64-K64（n = 8）、wzi 8-K8（n = 4）、wzi 37-K37（n = 4）、wzi 53-K53（n = 1）、wzi 125-K2（n = 1）和wzi 1-K1（n = 1）。不同类型CPS在生物被膜形成和吞噬功能方面存在差异。ST 11株的毒力低于其他ST株。KPC-Kp菌株表现出毒力相关性状的变异性。差异与ST类型和CPS有关。
Klebsiella pneumoniae is an important human pathogen associated with a variety of diseases and the prevalence of bla(KPC) carrying K. pneumoniae (KPC-Kp) is rapidly increasing. Capsule is an important virulence factor in K. pneumoniae. In this study, we determined to first systematically characterize capsular polysaccharide (CPS) and virulence traits in KPC-Kp strains. A total of 56KPC-Kp isolates were recovered from clinical samples in a Chinese hospital, which were assigned to clonal lineages by multilocus sequence typing (MLST). Capsule typing (wzi sequencing and wzc polymerase chain reaction [PCR]) and virulence genes were characterized by molecular approaches. The virulence of these strains was determined by biofilm formation, serum killing resistance, phagocytosis, and infection models. Six different STs were found among 56KPC-Kp isolates: 76.8% (43 of 56 isolates) belonged to ST11, 6 isolates belonged to ST147, 4 isolates belonged to ST15, 1 isolate belonged to ST1456, 1 isolate belonged to ST65, and 1 isolate was ST23. Based on the wzi gene DNA sequences and wzc PCR, these 56 strains were classified as capsular type wzi47-K47 (n = 37), wzi64-K64 (n = 8), wzi8-K8 (n = 4), wzi37-K37 (n = 4), wzi53-K53 (n = 1), wzi125-K2 (n = 1), and wzi1-K1 (n = 1). Heterogeneity was detected in biofilm formation and phagocytosis among different CPS types. ST11 strains were less virulent than other ST strains. KPC-Kp strains exhibit variability of virulence-associated traits. Differences were associated with the ST types and CPS.