A comparison of chemical pretreatment methods for improving saccharification of cotton stalks
A comparison of chemical pretreatment methods for improving saccharification of cotton stalks
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DOI：
10.1016/j.biortech.2006.10.022
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发表时间：
2007-11-01
影响因子：
11.4
通讯作者：
Osborne, Jason
中科院分区：
文献类型：
--
作者：
Silverstein, Rebecca A.;Chen, Ye;Osborne, Jason
研究了硫酸（H2SO 4）、氢氧化钠（NaOH）、过氧化氢（H2 O2）和臭氧预处理对棉秆转化为乙醇的影响。用浓度为0.5%、1%和2%（w/v）的H2SO 4、NaOH和H2 O2预处理固体负载量为10%（w/v）的磨碎的棉秆。处理温度为90摄氏度和121摄氏度，在15 psi的停留时间为30，60，和90分钟进行了研究。臭氧预处理进行在4摄氏度与恒定的鼓泡秸秆在水中。来自H2SO 4、NaOH和H2 O2预处理（在2%、60分钟、121 ℃/15 psi下）的固体显示出显著的木质素降解和/或高糖可用性，因此在50 ℃下被Celluclast 1.5 L和Novozym 188水解。硫酸预处理导致最高的木聚糖减少（对于2%酸，90分钟，121 ℃/15 psi为95.23%），但在水解期间纤维素至葡萄糖的转化率最低（23.85%）。氢氧化钠预处理导致最高水平的脱木质素（对于2%NaOH，90分钟，121 ℃/15 psi为65.63%）和纤维素转化率（60.8%）。过氧化氢预处理导致显著降低（p
The effectiveness of sulfuric acid (H2SO4), sodium hydroxide (NaOH), hydrogen peroxide (H2O2), and ozone pretreatments for conversion of cotton stalks to ethanol was investigated. Ground cotton stalks at a solid loading of 10% (w/v) were pretreated with H2SO4, NaOH, and H2O2 at concentrations of 0.5%, 1%, and 2% (w/v). Treatment temperatures of 90 degrees C and 121 degrees C at 15 psi were investigated for residence times of 30, 60, and 90 min. Ozone pretreatment was performed at 4 degrees C with constant sparging of stalks in water. Solids from H2SO4, NaOH, and H2O2 pretreatments (at 2%, 60 min, 121 degrees C/15 psi) showed significant lignin degradation and/or high sugar availability and hence were hydrolyzed by Celluclast 1.5 L and Novozym 188 at 50 degrees C. Sulfuric acid pretreatment resulted in the highest xylan reduction (95.23% for 2% acid, 90 min, 121 degrees C/15 psi) but the lowest cellulose to glucose conversion during hydrolysis (23.85%). Sodium hydroxide pretreatment resulted in the highest level of delignification (65.63% for 2% NaOH, 90 min, 121 degrees C/15 psi) and cellulose conversion (60.8%). Hydrogen peroxide pretreatment resulted in significantly lower (p