PROTHROMBIN MESSENGER-RNA IS EXPRESSED BY CELLS OF THE NERVOUS-SYSTEM
PROTHROMBIN MESSENGER-RNA IS EXPRESSED BY CELLS OF THE NERVOUS-SYSTEM
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DOI：
10.1016/0896-6273(91)90060-d
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发表时间：
1991-04-01
期刊：
影响因子：
16.2
通讯作者：
MONARD, D
中科院分区：
文献类型：
--
作者：
DIHANICH, M;KASER, M;MONARD, D
凝血酶是凝血系统中的一种丝氨酸蛋白酶，在体外对细胞有额外的作用。它是成纤维细胞和星形胶质细胞的有丝分裂原，并有助于调节轴突生长和星形胶质细胞星形。到目前为止，由于难以避免血清污染，凝血酶或其前体凝血酶原在肝脏以外的组织中的表达尚未得到确凿的证明。使用敏感的mRNA检测方法，我们在这里表明，凝血酶原不仅在肝脏中表达，而且在整个发育过程中在大脑中表达。聚合酶链式反应、Northern和原位杂交研究表明，在大鼠和人类神经系统的嗅球、皮质、小脑和其他区域以及神经细胞系中存在凝血酶原转录本。这些结果支持(前)凝血酶参与神经系统细胞事件的调节。
Thrombin, a serine protease of the blood coagulation system, has additional effects on cells in vitro. It is mitogenic for fibroblasts and astrocytes and contributes to the regulation of neurite outgrowth and astrocyte stellation. Until now the expression of thrombin or its precursor prothrombin in tissues other than liver has not been demonstrated conclusively because of difficulty in avoiding serum contamination. Using sensitive mRNA detection methods, we show here that prothrombin is expressed not only in the liver, but also in the brain throughout development. Polymerase chain reaction, Northern, and in situ hybridization studies demonstrate the presence of prothrombin transcripts in the olfactory bulb, the cortex, the cerebellum, and other regions of the rat and human nervous system, as well as in neural cell lines. These results support an involvement of (pro)thrombin in the regulation of cellular events in the nervous system.