Prevalence and characterization of Staphylococcus aureus and enterotoxigenic Staphylococcus aureus in retail raw chicken meat throughout Japan
Prevalence and characterization of Staphylococcus aureus and enterotoxigenic Staphylococcus aureus in retail raw chicken meat throughout Japan
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DOI：
10.1292/jvms.67.269
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发表时间：
2005-03-01
影响因子：
1.2
通讯作者：
Inamoto, T
中科院分区：
文献类型：
--
作者：
Kitai, S;Shimizu, A;Inamoto, T
在日本47个县的145家不同超市中销售的444份生鸡肉（鸡大腿、鸡胸、鸡翅、肝脏、鸡胗、心脏和卵巢）的样品被检测出是否含有金黄色葡萄球菌，并与金黄色葡萄球菌的肠毒性有关。其中292份（65.8%）检出金黄色葡萄球菌，145家超市中131家检出金黄色葡萄球菌。不同肉类的金黄色葡萄球菌检出率差异无统计学意义。714株分离株中约80%为禽（57.1%）和人（22.1%）生物型。从31个地市53家超市的78份样品中分离出360株产肠毒素的菌株78株（21.7%）；产生的葡萄球菌肠毒素（SEs）为SEB（50株）、SEA（14株）、SEC（8株）、SED（2株）、SEA+SEB（2株）和SEA+SEC（2株）。大多数产肠毒素的分离物属于人类和家禽的生物型，凝固酶型为VII、VIII或IV型，被III类噬菌体裂解。从同一超市的不同种类的肉类分离物、从同一县的不同超市采集的样品或从几个不同县采集的样品分离物中发现相同的SE型、生物型、凝固酶型和脉冲场凝胶电泳（PFGE）模式。在50株产生seb的分离株中，27株产生了3种相似的PFGE模式，差异仅为几个片段，表明它们在遗传上密切相关。在11个县的17家超市零售的分离样品中发现了这三种模式，表明它们可能在日本的生鸡肉中传播。
A total of 444 samples of raw chicken meat (thighs, breasts, wings, livers, gizzards, hearts and ovaries) that retailed at 145 different supermarkets in 47 prefectures in Japan were examined for contamination with Staphylococcus aureus in association with its enterotoxigenicity. S. aureus was isolated from 292 (65.8%) of the samples, and from 131 of the 145 supermarkets. There was no significant difference in the detection rate of S. aureus according to the type of meat examined. About 80% of 714 isolates belonged to the poultry (57.1%) and human biotypes (22.1%). Seventy-eight (21.7%) of 360 isolates were enterotoxigenic and isolated from 78 samples in 53 supermarkets in 31 prefectures. Staphylococcal enterotoxins (SEs) produced were SEB (50 isolates), SEA (14), SEC (8), SED (2), SEA+SEB (2), and SEA+SEC (2). Most of the enterotoxigenic isolates belonged to the human and poultry biotypes, coagulase type VII, VIII or IV, and were lysed by phages of group III. Identical SE types, biotypes, coagulase types and pulsed-field gel electrophoresis (PFGE) patterns were shown in isolates from different types of meat at the same supermarket and from samples taken from different supermarkets in the same prefectures or in isolates from samples obtained from several different prefectures. Among the 50 SEB-producing isolates, 27 yielded three similar PFGE patterns that differed by only a few fragments, suggesting that they were closely related genetically. The three patterns were found in isolates of samples that retailed at 17 supermarkets in 11 prefectures, indicating that they may be disseminated among raw chicken meat in Japan.