SOLUBLE SUPEROXIDE-DISMUTASE (SOD) IN SUSCEPTIBLE AND RESISTANT HOST-PARASITE COMPLEXES OF PHASEOLUS-VULGARIS AND UROMYCES-PHASEOLI
SOLUBLE SUPEROXIDE-DISMUTASE (SOD) IN SUSCEPTIBLE AND RESISTANT HOST-PARASITE COMPLEXES OF PHASEOLUS-VULGARIS AND UROMYCES-PHASEOLI
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DOI：
10.1016/0885-5765(87)90062-2
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发表时间：
1987-09-01
影响因子：
2.7
通讯作者：
MONTALBINI, P
中科院分区：
文献类型：
--
作者：
BUONAURIO, R;DELLATORRE, G;MONTALBINI, P
测定了感病菜豆叶片中可溶性超氧化物歧化酶（EC 1.15.1.1）的含量。平托111）和过敏抗性（cvs K.W. 765和K.W. 914)对菜豆单胞锈菌（Uromyces phaseoli）的抗性。在所有的品种测试中，提取的超氧化物歧化酶（SOD）的水平增加似乎与外观florescent和分化的夏堆，在敏感品种，和微观或宏观坏死病变的过敏品种。相容和不相容的宿主-病原体相互作用选择性地刺激不同的SOD酶（Mn或Cu、Zn-SOD）。在叶肉基质分化过程中，感病叶片中锰酶活性增加。与此相反，有增加的Cu，Zn-酶的活性在过敏反应的抗性叶片。上述增加是由于宿主对感染的反应，因为真菌SOD的贡献可以忽略不计。SOD和过氧化物酶同时升高。只有在感病品种中过氧化物酶的增加量大大超过SOD。感染没有改变SOD酶的电泳行为。
The level of soluble superoxide dismutase (EC 1.15.1.1) was determined in Phaseolus vulgaris leaves susceptible (cv. Pinto 111) and hypersensitive resistant (cvs K.W. 765 and K.W. 914) to Uromyces phaseoli. In all cultivars tested increased levels of extracted superoxide dismutase (SOD) appeared to be associated with the appearance of flecks and the differentiation of the uredosori, in the susceptible cultivar, and of microscopic or macroscopic necrotic lesions in the hypersensitive cultivars. Different SOD enzymes (Mn or Cu, Zn-SOD) were selectively stimulated by compatible and incompatible host-pathogen interactions. There was an increase in activity of the Mn-enzyme in the susceptible leaves during stroma differentiation in the mesophyll. By contrast there was an increase in activity of the Cu,Zn-enzyme in the hypersensitive response of resistant leaves. The above mentioned increases were due to a host response to infection since the contribution of fungal SOD was negligible. SOD and peroxidase increased simultaneously. The increase of peroxidase greatly exceeded that of SOD only in the hypersensitive cultivars. The infection did not change the electrophoretic behaviour of SOD enzymes.