Fluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism
Fluorescence Probing Live Single-cell Mass Spectrometry for Direct Analysis of Organelle Metabolism
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DOI：
10.2116/analsci.31.1211
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发表时间：
2015-12-01
影响因子：
1.6
通讯作者：
Masujima, Tsutomu
中科院分区：
文献类型：
--
作者：
Esaki, Tsuyoshi;Masujima, Tsutomu
用荧光探针观察活细胞中的线粒体，以确定它们在活细胞中的位置和状态。然后，在荧光显微镜下用纳米螺旋尖端选择性地捕获线粒体，发现了数千个小分子峰，并发现了线粒体特有的类固醇。这种荧光成像与活的单细胞质谱学相结合，为分析特定部位和状态的分子检测打开了大门，以阐明单细胞和细胞器水平上的精确分子机制。
Mitochondria in a live HepG2 cell were visualized with a fluorescent probe to specify their location and state in a living cell. Then, mitochondria were selectively captured with a nanospray tip under fluorescence microscope, and thousands of small molecular peaks were revealed and unique steroids specific to mitochondria were also found. This fluorescence imaging combined with live single-cell mass spectrometry opens the door to the analysis of site- and state-specific molecular detection to elucidate precise molecular mechanisms at the single-cell and organelle level.