Characterisation of the immunoglobulin variable region gene usage encoding the murine anti-ganglio side antibody repertoire
Characterisation of the immunoglobulin variable region gene usage encoding the murine anti-ganglio side antibody repertoire
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DOI：
10.1016/j.jneuroim.2005.04.011
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发表时间：
2005-08-01
影响因子：
3.3
通讯作者：
Willison, HJ
中科院分区：
文献类型：
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作者：
Boffey, J;Odaka, M;Willison, HJ
与末端二唾液酸半乳糖表位发生反应的神经病性鼠抗体是固有的，并且优先由 VH7183.3b 基因编码。在这里，我们研究了与内部半乳糖连接的二唾液酸基表位和 GT1b 末端三糖反应的抗体。抗体具有中等亲和力且未突变。抗 GD1b 抗体通常由 VH10.2b 重链和 gi38e 轻链基因编码。具有更广泛聚糖结合模式的抗 GT1b 抗体由 VHQ52 和 VHJ558 家族基因编码。这些数据表明，神经节苷脂结合抗体的离散特异性是由先天 B 细胞库中 V 基因使用的特定模式决定的。 (C) 2005 Elsevier B.V. 保留所有权利。
Neuropathogenic murine antibodies reactive with terminal disialylgalactose epitopes are innate and preferentially encoded by the VH7183.3b gene. Here we have studied antibodies reactive with internal galactose-linked disialosyl epitopes and the terminal trisaccharide of GT1b. Antibodies were of moderate affinity and unmutated. Anti-GD1b antibodies were often encoded by the VH10.2b heavy and gi38e light chain genes. Anti-GT1b antibodies with broader glycan binding patterns were encoded by VHQ52 and VHJ558 family genes. These data indicate that the discrete specificities of ganglioside-binding antibodies are dictated by particular patterns of V gene usage residing within the innate B cell repertoire. (C) 2005 Elsevier B.V. All rights reserved.