Extravascular optical coherence tomography: evaluation of carotid atherosclerosis and pravastatin therapy.
Extravascular optical coherence tomography: evaluation of carotid atherosclerosis and pravastatin therapy.
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DOI：
10.1161/strokeaha.113.002970
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发表时间：
2014-04
期刊：
影响因子：
8.3
通讯作者：
Kang JU
中科院分区：
文献类型：
--
作者：
Wicks RT;Huang Y;Zhang K;Zhao M;Tyler BM;Suk I;Hwang L;Ruzevick J;Jallo G;Brem H;Pradilla G;Kang JU
血管外光学相干断层扫描（OCT），作为一种非侵入性的成像方法与微米分辨率，评价了在小鼠模型的颈动脉粥样硬化的普伐他汀治疗的疗效。OCT器械设计用于血管外斑块成像。将野生型小鼠和载脂蛋白E缺陷（ApoE-/-）小鼠随机分为三个治疗组：1）野生型，标准啮齿动物饲料（n=13）; 2）ApoE-/-，高脂肪动脉粥样硬化饮食（HFD）（n=13）; 3）ApoE-/-，HFD，每日给予普伐他汀（n=13）。将小鼠麻醉并手术暴露左颈总动脉。评价了血管腔通畅性的3D（2个空间维度+时间）和4D（3个空间维度+时间）OCT图像。灌注后，进行原位OCT成像，与体内结果和最终组织学进行统计学比较。术中OCT成像在100%接受HFD的ApoE-/-小鼠中阳性识别出颈动脉斑块。与野生型小鼠相比，接受HFD的ApoE-/-小鼠具有显著降低的管腔通畅性（p<0.001）。发现普伐他汀治疗显著增加HFD的ApoE-/-小鼠的管腔通畅性（与HFD的ApoE-/-相比，p<0.01）。灌注后OCT成像和组织学检查证实了这些发现。OCT成像提供了实时、详细血管管腔评价的可能性，可能改善脑血管神经外科手术期间的手术准确性和结局。普伐他汀显著增加ApoE-/-小鼠HFD的血管腔通畅性。
Extravascular optical coherence tomography (OCT), as a non-invasive imaging methodology with micrometer resolution, was evaluated in a murine model of carotid atherosclerosis by way of assessing the efficacy of pravastatin therapy. An OCT device was engineered for extravascular plaque imaging. Wild-type mice and apolipoprotein E-deficient (ApoE-/-) mice were randomized to three treatment groups: 1) wild-type on a diet of standard rodent chow (n=13); 2) ApoE-/- on a high-fat, atherosclerotic diet (HFD) (n=13); and 3) ApoE-/- on a HFD given daily pravastatin (n=13). Mice were anesthetized and the left common carotid was surgically exposed. 3D (2 spatial dimensions+time) and 4D (3 spatial dimensions+time) OCT images of the vessel lumen patency were evaluated. After perfusion, in situ OCT imaging was performed for statistical comparison with the in vivo results and final histology. Intraoperative OCT imaging positively identified carotid plaque in 100% of ApoE-/- mice on HFD. ApoE-/- mice on HFD had a significantly decreased lumen patency compared with wild-type mice (p<0.001). Pravastatin therapy was found to significantly increase lumen patency in ApoE-/- mice on HFD (p<0.01, compared with ApoE-/- on HFD). The findings were confirmed with OCT imaging after perfusion and histology. OCT imaging offers the potential for real-time, detailed vessel lumen evaluation, potentially improving surgical accuracy and outcomes during cerebrovascular neurosurgical procedures. Pravastatin significantly increases vessel lumen patency in the ApoE-/- mouse on HFD.