Snapshot of degenerative aging of porcine intervertebral disc: a model to unravel the molecular mechanisms
Snapshot of degenerative aging of porcine intervertebral disc: a model to unravel the molecular mechanisms
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DOI：
10.3858/emm.2011.43.6.036
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发表时间：
2011-06-30
影响因子：
12.8
通讯作者：
Kim, Song-Ja
中科院分区：
文献类型：
--
作者：
Cho, Hongsik;Park, Sang-Hyug;Kim, Song-Ja
就生物力学和生物化学而言，较大的动物模型（例如猪）已被验证为人类椎间盘的合适模型。它们对于研究是有利的，因为模型的准备相对简单，并且很容易获得以进行外科技术的研究。本研究的目的是定量分析不同年龄（新生儿；2-3周，成熟；6-9个月，老年；2-3岁）猪椎间盘中胶原蛋白和细胞外基质蛋白聚糖成分以及胶原酶（MMP-1）的基因表达。在这项研究中，我们观察到细胞数量和 GAG（糖胺聚糖）形成随着衰老而急剧减少。此外，纤维环（AF）和髓核（NP）细胞中的基因表达随着衰老而变化。 MMP-1 mRNA 水平随年龄增加而增加，I 型、II 型胶原蛋白水平随年龄增加而减少。聚集蛋白聚糖 mRNA 水平在成熟组中最高，并随着年龄的增长而显着下降。与新生儿组相比，成熟组中 MMP-1 的表达最少。在AF细胞中，成熟组的II型胶原蛋白表达水平较高，聚集蛋白聚糖水平较高，而老化的NP则显示II型胶原蛋白减少。猪椎间盘的 IVD 退变模型显示出许多基因表达随年龄变化的变化，这些变化之前已在人类中记录过，并且可以作为研究 IVD 代谢随年龄变化的模型。我们得出的结论是，猪模型非常适合测试与椎间盘退变相关的假设，同时允许在生物活性系统中进行时间过程研究。
Larger animal models, such as porcine, have been validated as appropriate models of the human disc with respect to biomechanics and biochemistry. They are advantageous for research as the models are relatively straightforward to prepare and easily obtainable for research to perform surgical techniques. The intention of this study was to quantitatively analyze gene expression for collagen and proteoglycan components of the extracellular matrix and for collagenase (MMP-1) in porcine discs of varying ages (Newborn; 2-3weeks, Mature; 6-9 month, Older; 2-3 years). In this study, we observed that the cell number and GAG (glycosaminoglycan) formation dramatically decreased with aging. Also, gene expression in the annulus fibrosus (AF) and nucleus pulposus (NP) cells changed with aging. The level of MMP-1 mRNA increased with age and both type I, II collagens decreased with age. The level of aggrecan mRNA was highest in the mature group and decreased significantly with aging. In the mature group, MMP-1 expression was minimal compared to the newborn group. In AF cells, type II collagen was expressed at a high level in the mature group with a higher level of aggrecan, when aged NP showed a decrease in type II collagen. The model of IVD degeneration in the porcine disc shows many changes in gene expression with age that have been previously documented for human and may serve as a model for studying changes in IVD metabolism with age. We concluded that the porcine model is excellent to test hypotheses related to disc degeneration while permitting time-course study in biologically active systems.