Salting-out assisted solvent extraction of L (+) lactic acid obtained after fermentation of sugarcane bagasse hydrolysate
Salting-out assisted solvent extraction of L (+) lactic acid obtained after fermentation of sugarcane bagasse hydrolysate
复制标题
DOI：
10.1016/j.seppur.2021.118788
复制
发表时间：
2021-04-23
影响因子：
8.6
通讯作者：
Agrawal, Deepti
中科院分区：
文献类型：
--
作者：
Baral, Pratibha;Pundir, Anushka;Agrawal, Deepti
乳酸是由木质纤维素生物质等廉价且可再生的原料生产的十二种平台化学品之一。本研究阐述了盐析辅助溶剂萃取法提取源自甘蔗渣水解物的L(+) - 乳酸。对各种萃取剂和稀释剂的筛选研究表明，磷酸三正丁酯和乙酸乙酯的组合效果最佳，从调节至pH为1.6 ± 0.2的稀发酵液中提取出了59.63 ± 1.28%的乳酸。对各种无机盐进行了筛选以进一步提高萃取效率。添加60%（w/v）的硫酸铵使乳酸萃取率提高了36.17%。在优化条件下，该盐浓度成功地从pH约为2.5、含40 - 100 g/L乳酸的发酵液中向有机相提取了85.95 ± 0.44%的乳酸。还表明使用冷却的丙酮可回收>80%的盐，该盐在重复使用时，在萃取过程中仅有3.3%的轻微下降。因此，本研究展示了一种在温和操作条件下使用乙酸乙酯等绿色溶剂的环保方法。
Lactic acid is among the twelve platform chemicals produced from inexpensive and renewable feedstocks such as lignocellulosic biomass. The present study illustrates salting-out assisted solvent extraction of L (+) lactic acid, derived from sugarcane bagasse hydrolysate. Screening studies with various extractants and diluents revealed that a combination of tri-n-butyl phosphate and ethyl acetate yielded the best results and extracted 59.63 +/- 1.28% lactic acid from the dilute fermentation broth adjusted to a pH of 1.6 +/- 0.2. Various inorganic salts were screened to enhance extraction efficiency further. The addition of 60% (w/v) ammonium sulfate improved the lactic acid extraction by 36.17%. This salt concentration successfully extracted 85.95 +/- 0.44% lactic acid to the organic phase under optimized conditions from the fermentation broth at a pH of similar to 2.5 containing 40-100 g L-1 lactic acid. Recovery of > 80% salt is also shown using chilled acetone, which upon reuse showed a nominal decline of 3.3% during extraction. Thus an eco-friendly approach using a green solvent like ethyl acetate with mild operating conditions is demonstrated in the present study.