Determination of sugar phosphates and nucleotides related to photosynthetic metabolism by high-performance anion-exchange liquid chromatography with fluorometric and ultraviolet detection.
Determination of sugar phosphates and nucleotides related to photosynthetic metabolism by high-performance anion-exchange liquid chromatography with fluorometric and ultraviolet detection.
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采用荧光和紫外检测的高效阴离子交换液相色谱法测定与光合代谢相关的糖磷酸盐和核苷酸。
DOI：
10.1016/s0003-2697(02)00644-9
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发表时间：
2003
影响因子：
2.9
通讯作者：
M. Kasai
中科院分区：
文献类型：
--
作者：
S. Sawada;R. Ono;Takayuki Sato;Satoshi Suzuki;O. Arakawa;M. Kasai
建立了高效阴离子交换液相色谱系统，用于鉴定光合代谢中的糖、磷酸和核苷酸。首先用硼酸和磷酸钠梯度洗脱分离磷酸糖和核苷酸，然后分别用荧光检测器（作为精氨酸的荧光衍生物）和紫外检测器检测。使用该系统可以分析8种真实的糖磷酸和11种真实的核苷酸。该系统的适用性，以测定相应的糖磷酸和核苷酸提取物中只有五个大豆叶圆片（8.95厘米2）。
A high-performance anion-exchange liquid chromatography system was constructed to identify sugar phosphates and nucleotides involved in photosynthetic metabolism. First sugar phosphates and nucleotides were separated by a gradient elution with boric acid and sodium phosphate, then they were detected by a fluorescence detector (as fluorescent derivatives with arginine) and UV detector, respectively. Eight authentic sugar phosphates and 11 authentic nucleotides could be analyzed using the system. The applicability of the system to the determination of the corresponding sugar phosphates and nucleotides in extracts from only five soybean leaf discs (8.95cm2) was shown.