Development of a microfluidic device for fluorescence activated cell sorting
Development of a microfluidic device for fluorescence activated cell sorting
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DOI：
10.1088/0960-1317/12/4/324
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发表时间：
2002-07-01
影响因子：
2.3
通讯作者：
O'Brien, P
中科院分区：
文献类型：
--
作者：
Krüger, J;Singh, K;O'Brien, P
本文描述了一种小型化的分析系统的发展，它可以执行流式细胞仪的主要关键功能。开发的目标是用于细胞计数和分选的诊断应用，最终目标是一种低成本的便携式护理点诊断仪器。目前的系统配置由一次性微流控装置组成，该装置能够通过小型化的激光诱导荧光检测系统进行注射、单个文件单元流动以及对识别的样品进行分选。基于厚膜光聚合物的快速成型技术制备了微流控器件。本文报道了细胞分选的各种方法，并演示了利用片外阀门开关技术对单个细胞进行分选的方法。微型荧光检测系统采用主动和被动微型光学元件，包括半导体激光器和超高亮度LED光源、高灵敏度雪崩光电二极管以及用于传输和收集光的微棱镜、全息衍射光栅和光纤。此外，我们还论证了将固态组件作为片上检测系统的一部分的可行性。
This paper describes the development towards a miniaturized analytical system that can perform the major key functions of a flow cytometer. The development aims at diagnostic applications for cell counting and sorting with the ultimate goal of a low-cost portable instrument for point of Care diagnosis. The present systems configuration consists of a disposable microfluidic device, that enables injection, single file cell flow through a miniaturized laser induced fluorescence detection system as well as sorting of identified samples. The microfluidic devices were fabricated by means of rapid prototyping technologies based on thick film photo-polymers. This paper reports various approaches on cell sorting and demonstrates sorting of single cells by means of an off-chip valve switching technique. The miniaturized fluorescence detection system employs active and passive micro-optical components, including semiconductor laser and ultra bright LED sources, highly sensitive avalanche photodiodes as well as micro-prism, holographic diffraction gratings and Fibre optics for transmission and collection of light. Furthermore we demonstrate the feasibility of integrating solid-state components as part of an on-chip detection system.