NEW CONSISTENT CHROMOSOMAL ABNORMALITY IN CHRONIC MYELOGENOUS LEUKEMIA IDENTIFIED BY QUINACRINE FLUORESCENCE AND GIEMSA STAINING
NEW CONSISTENT CHROMOSOMAL ABNORMALITY IN CHRONIC MYELOGENOUS LEUKEMIA IDENTIFIED BY QUINACRINE FLUORESCENCE AND GIEMSA STAINING
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DOI：
10.1038/243290a0
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发表时间：
1973-01-01
期刊：
影响因子：
64.8
通讯作者：
ROWLEY, JD
中科院分区：
文献类型：
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作者：
ROWLEY, JD
连续9例慢性髓性白血病（CML）患者的细胞用奎纳克里荧光和各种吉姆萨染色技术进行了分析。所有9例患者的费城（Ph1）染色体均缺失22号染色体长臂（22q−）1,2。这些新的染色技术在9名患者的所有细胞中发现了一种意想不到的异常。它由一条9号染色体（9q+）长臂末端添加的暗淡荧光物质组成。在giemsa染色的制备中，这种物质在一条9号染色体上显示为一个附加的微弱末端带。额外物质的数量大约等于Ph1（22q−）染色体缺失的数量，这表明在22号长臂和9号长臂之间可能存在迄今未被发现的易位，产生了9q+染色体。
CELLS from nine consecutive patients with chronic myelogenous leukaemia (CML) have been analysed with quinacrine fluorescence and various Giemsa staining techniques. The Philadelphia (Ph1) chromosome in all nine patients represents a deletion of the long arm of chromosome 22 (22q−)1,2. An unsuspected abnormality in all cells from the nine patients has been detected with these new staining techniques. It consists of the addition of dully fluorescing material to the end of the long arm of one chromosome 9 (9q+). In Giemsa-stained preparations, this material appears as an additional faint terminal band in one chromosome 9. The amount of additional material is approximately equal to the amount missing from the Ph1(22q−) chromosome, suggesting that there may be a hitherto undetected translocation between the long arm of 22 and the long arm of 9, producing the 9q+ chromosome.