Development and validation of an LC-MS/MS method for simultaneous quantitative analysis of free and conjugated bisphenol A in human urine
Development and validation of an LC-MS/MS method for simultaneous quantitative analysis of free and conjugated bisphenol A in human urine
复制标题
DOI：
10.1002/bmc.3090
复制
发表时间：
2014-05-01
影响因子：
1.8
通讯作者：
Tunctan, Bahar
中科院分区：
文献类型：
--
作者：
Battal, Dilek;Cok, Ismet;Tunctan, Bahar
双酚A（BPA）是一种环境内分泌干扰物，广泛用于普通消费品中。由于双酚A的雌激素活性可能产生不良影响，人们越来越关注人类接触双酚A的问题。为了评估BPA的环境暴露，必须有一种灵敏、准确和选择性的分析方法，特别是可以检测复杂样品基质中低水平BPA的方法。在本研究中，我们开发并验证了一种准确、灵敏和耐用的液相色谱-串联质谱法，用于仅通过单次注射同时定量人尿液中的游离BPA和BPA-d-葡糖苷酸（BPA-gluc）浓度。BPA和BPA-gluc的线性范围分别为1- 100 ng/mL和10- 1000 ng/mL。采用高效液相色谱-电喷雾串联质谱法（HPLC/ESI-MS/MS），在选择离子监测模式下，以五氟苯基丙基柱为分离柱，对样品进行定量分析。将验证的方法应用于从随机选择的健康人受试者中采集的点尿标本的分析。版权所有（c）2013约翰威利父子有限公司
Bisphenol A (BPA) is an environmental endocrine-disrupting chemicals that is widely used in common consumer products. There is an increasing concern regarding human exposure to BPA owing to the potential adverse effects associated with its estrogenic activity. For assessing environmental exposure to BPA, it is essential to have a sensitive, accurate and selective analytical method, especially one that can detect low BPA levels in complex sample matrices. In this study, we developed and validated an accurate, sensitive, and robust liquid chromatography-tandem mass spectrometry method for simultaneous quantification of free BPA and BPA -d-glucuronide (BPA-gluc) concentrations in human urine with only a single injection. Calibration curves were linear over a concentration range of 1-100ng/mL for BPA and 10-1000ng/mL for BPA-gluc. The levels of the analytes were determined quantitatively with HPLC/ESI-MS/MS by using negative electrospray ionization in the select ion monitoring mode and a pentaflouraphenyl propyl column. The validated method was applied to the analysis of spot urine specimens collected from randomly selected healthy human subjects. Copyright (c) 2013 John Wiley & Sons, Ltd.