Extracellular heat shock protein 70 promotes osteogenesis of human mesenchymal stem cells through activation of the ERK signaling pathway
Extracellular heat shock protein 70 promotes osteogenesis of human mesenchymal stem cells through activation of the ERK signaling pathway
复制标题
细胞外热休克蛋白70通过激活ERK信号通路促进人间充质干细胞的成骨。
DOI：
10.1016/j.febslet.2015.11.021
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发表时间：
2015-12-21
期刊：
影响因子：
3.5
通讯作者：
Pan, Zhijun
中科院分区：
文献类型：
--
作者：
Chen, Erman;Xue, Deting;Pan, Zhijun
当细胞受到压力时，热休克蛋白具有保护作用。然而，细胞外热休克蛋白70 （eHSP70）与hMSCs成骨的关系尚未见报道。结果表明，HSP70 （200 ng/ml）可提高碱性磷酸酶活性，促进hMSC矿化。在成骨诱导条件下，HSP70显著上调runt家族转录因子Runx2和osterix （OSX）等骨特异性基因的表达。通过微阵列和通路分析比较表达谱显示，HSP70通过激活ERK信号通路促进hMSCs成骨。HSP70可能是治疗骨不连的潜在治疗剂。(C) 2015年欧洲生化学会联合会。Elsevier B.V.版权所有。
Heat shock proteins have protective effects when cells are exposed to stress. However, the relationship between extracellular heat shock protein 70 (eHSP70) and osteogenesis of hMSCs has not been reported. The results of this study showed that HSP70 (200 ng/ml) increases alkaline phosphatase activity and promotes hMSC mineralization. Under osteogenic induction conditions, HSP70 significantly upregulated the expression of osteo-specific genes, such as the runt family transcription factor Runx2 and osterix (OSX). Comparative expression profiling by microarray and pathway analyses revealed that HSP70 promotes osteogenesis of hMSCs through activation of the ERK signaling pathway. HSP70 may be a potential therapeutic agent for the treatment of bone nonunion. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.