Molecular Identification of Aspergillus Species Collected for the Transplant-Associated Infection Surveillance Network
Molecular Identification of Aspergillus Species Collected for the Transplant-Associated Infection Surveillance Network
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DOI：
10.1128/jcm.01070-09
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发表时间：
2009-10-01
影响因子：
9.4
通讯作者：
Chiller, Tom
中科院分区：
文献类型：
--
作者：
Balajee, S. Arunmozhi;Kano, Rui;Chiller, Tom
从移植相关感染监测网络（一项为期6年的前瞻性监测研究）中获得了来自已证实或可能患有侵袭性曲霉病的移植患者的大量曲霉菌临床分离株（n = 218）。为了确定该集合中的曲霉属物种分布，通过使用内部转录间隔区和β-微管蛋白区域对分离株进行比较序列分析。烟曲霉为优势种，其次为A.黄曲霉A.尼日尔.鉴定了几个新记录种，包括A. lentulus和A. calidoustus;这两个物种有高的体外MIC多种抗真菌药物。塔宾曲霉（Aspergillus tubingensis）是A.尼日尔种复合体，从临床标本中描述;塔宾分离株对抗真菌药物的体外MIC较低。
A large aggregate collection of clinical isolates of aspergilli (n = 218) from transplant patients with proven or probable invasive aspergillosis was available from the Transplant-Associated Infection Surveillance Network, a 6-year prospective surveillance study. To determine the Aspergillus species distribution in this collection, isolates were subjected to comparative sequence analyses by use of the internal transcribed spacer and beta-tubulin regions. Aspergillus fumigatus was the predominant species recovered, followed by A. flavus and A. niger. Several newly described species were identified, including A. lentulus and A. calidoustus; both species had high in vitro MICs to multiple antifungal drugs. Aspergillus tubingensis, a member of the A. niger species complex, is described from clinical specimens; all A. tubingensis isolates had low in vitro MICs to antifungal drugs.